核农学报2024，Vol.38Issue(4)：622-632,11.DOI:10.11869/j.issn.1000-8551.2024.04.0622
红蓝光诱导下红地球葡萄VvBES1-1基因表达模式分析
Gene Expression Patterns of VvBES1-1 in Red Globe Grapes Induced by Red and Blue Lights
摘要
Abstract
BRI1-EMS-Suppressor 1(BES1),a crucial transcription factor involved in oleuropein lactone signaling,has been demonstrated to play a significant involvement in the regulation of plant photomorphogenesis and bud development.In order to investigate whether VvBES1 regulates flower bud differentiation through responding to red and blue light in Red Globe grapes,as well as the expression pattern of VvBES1 under this mode of regulation.In this study,red globe grape buds were utilized as test materials,while a 4:1 ratio of red and blue light(R4B1)was used as the light treatment and greenhouse natural light served as the control(CK).Through screening from transcriptome data generated before,VvBES1-1(Vitvi10g00636)was selected,and its coding sequence(CDS)was obtained via homologous cloning with a total length of 1 026 bp,encoding 341 amino acids.Bioinformatics analysis revealed that VvBES1-1 protein contained one highly conserved BES1-N binding structural domain of the BES1 family,belonging to the same subfamily as riverbank grapes.The expression pattern of VvBESl-1 showed tissue-specificity and continuous expression throughout all periods;However,its expression during R4B1 treatment period was significantly lower than that under natural light conditions.On September 15th,VvBES1-1 reached its highest expression level,coinciding with the developmental phase of secondary axis in red globe grapevine inflorescence.Therefore,it is hypothesized that VvBES1-1 plays a crucial role during this developmental period.Tobacco heterologous transformation assay demonstrated delayed flowering and shortened internodes in transgenic plants expressing VvBES1-1 gene,indicating altered phenotypic traits.Upon treatment with exogenous BR,the expression of the VvBES1-1 gene was upregulated.The expression of VvBES1-1 was increased first and then decreased,which was consistent with the light-mediated regulation of floral differentiation in Red Globe grapevine.In conclusion,this study provides a theoretical framework elucidating the role of VvBES1-1 in red and blue light-mediated BR signaling for controlling flower bud differentiation in the Red Globe grape.关键词
红地球葡萄/VvBES1-1/BR信号/表达特性Key words
red globe grapes/VvBES1-1/BR signaling expression properties引用本文复制引用
党仕卓,周娟,汤学燊,刘鑫,张亚红,袁苗..红蓝光诱导下红地球葡萄VvBES1-1基因表达模式分析[J].核农学报,2024,38(4):622-632,11.基金项目
宁夏回族自治区重点研发计划项目(2021BEF02016) （2021BEF02016）
