核农学报2024，Vol.38Issue(4)：662-673,12.DOI:10.11869/j.issn.1000-8551.2024.04.0662
转录组和蛋白组联合揭示甜瓜响应涝害胁迫的分子机制
Joint Analysis of Transcriptome and Proteome Revealed the Molecular Mechanism of Melon(Cucumis melo L.)in Response to Waterlogging Stress
摘要
Abstract
To investigate the molecular mechanism of melon(Cucumis melo L.)in response to waterlogging stress,we conducted a comparative analysis of the transcriptome and proteome of the waterlogging-sensitive accession L39 and the waterlogging-tolerant accession L45 under normal water supply and waterlogging stress conditions.In the leaves of waterlogging-stressed L39,a total of 3 532 genes showed differential expression compared to the control,while in L45,1 842 genes showed differential expression.Additionally,we identified 105 and 38 differentially expressed proteins in the comparison groups L39-W vs L39-C and L45-W vs L45-C,respectively.Comparing the transcriptome and proteome datasets,we observed that 38 and 12 genes exhibited jointly up-regulated and down-regulated expression profiles at the mRNA and protein levels in the L39-W vs L39-C comparison,whereas 7 genes showed co-up-regulated expression and three genes displayed co-down-regulated expression in the L45-W vs L45-C comparison.The KEGG pathway analysis revealed that the co-up-regulated genes in the L39-W vs L39-C comparison were mainly involved in'flavonoid biosynthesis','phenylpropanoid biosynthesis','phenylalanine metabolism','valine,leucine and isoleucine biosynthesis',and'arginine and proline metabolism'.In contrast,the co-up-regulated pathways in the L45-W vs L45-C comparison were predominantly related to'α-linolenic acid metabolism','flavonoid biosynthesis','phenylpropanoid biosynthesis',and'linoleic acid metabolism'.By combining transcriptome and proteome data,this study provides a foundation for further exploration of key genes and pathways involved in the response of melon to waterlogging stress.关键词
甜瓜/涝害胁迫/转录组/蛋白组Key words
Cucumis melo/waterlogging stress/transcriptome/proteome引用本文复制引用
张焕欣,闫承璞,李国权,张新龙,曹娜,乐美旺,朱方红,刘文革..转录组和蛋白组联合揭示甜瓜响应涝害胁迫的分子机制[J].核农学报,2024,38(4):662-673,12.基金项目
国家自然科学基金项目(32260756),中国博士后科学基金(2021M701514),江西省博士后科研择优资助项目(2021KY44),江西省农业科学院基础研究与人才培养专项资助项目(JXSNKYJCRC202311) （32260756）
