解剖学杂志2023，Vol.46Issue(6)：476-483,544,9.DOI:10.3969/j.issn.1001-1633.2023.06.003
兔膝关节前交叉韧带和内侧副韧带损伤后差异表达的内源性竞争性lncRNA和mRNA网络与核心基因的筛选及功能分析
Screening and functional analysis of differentially expressed endogenous competitive lncRNA and mRNA networks and core genes after anterior cruciate ligament and medial collateral ligament injuries in knee joint of rabbits
摘要
Abstract
Objective:To compare the differential expression of endogenous competitive long-chain non-coding ribonucleic acid(lncRNA)with messenger ribonucleic acid(mRNA)before and after partial injury of the anterior cruciate ligament(ACL)and medial collateral ligament(MCL)in knee joint of rabbits and to explore the possible molecular mechanisms impeding the endogenous repair of the ACL.Methods:RNA after partial injury of ACL and MCL in rabbits was sequenced to obtain differentially expressed lncRNAs and differentially expressed mRNAs and verified by qRT-PCR,followed by Kyoto Encyclopaedia of Genes and Genomes(KEGG)and Gene Ontology(GO)analyses for the construction of a ceRNA network.Results:A total of 853 differential lncRNAs and 994 differential mRNAs were obtained.GO and KEGG analyses showed that the differential lncRNAs differed significantly in inflammatory responses,extracellular matrix-receptor interactions,and cell proliferation,and the differential mRNAs differed significantly in cytokine activation and cell adhesion.Significant differences in core genes were found between the ACL and the MCL after partial injury.Conclusion:Significant differences in lncRNAs and mRNAs between partially injured ACL and MCL in rabbits provide more theoretical basis for the molecular level study of the mechanism of endogenous repair disorders in ACL injury.关键词
膝关节/前交叉韧带/内侧副韧带/长链非编码RNA/生物信息学/兔Key words
knee joint/anterior cruciate ligament/medial collateral ligament/long non-coding RNA/bioinformatics/rabbit引用本文复制引用
于涵,李一帆,李彬,温昱..兔膝关节前交叉韧带和内侧副韧带损伤后差异表达的内源性竞争性lncRNA和mRNA网络与核心基因的筛选及功能分析[J].解剖学杂志,2023,46(6):476-483,544,9.基金项目
沈阳市科学计划项目(20-205-4-078) （20-205-4-078）
