解剖学杂志2023，Vol.46Issue(6)：492-496,5.DOI:10.3969/j.issn.1001-1633.2023.06.005
高原低氧下妊娠大鼠胎盘葡萄糖转运体1的表达及其对胎鼠生长发育的影响
Expression of placental glucose transporter 1 in pregnant rats under plateau hypoxia and its effect on fetal rat growth and development
摘要
Abstract
Objective:To analyze the expression of glucose transporter 1(GLUT1)in placental tissues of pregnant rats under plateau hypoxia and its effect on the growth and development of fetal rats.Methods:Twenty pregnant rats were randomly divided into normoxic group and hypoxic group.On the 9th day of pregnancy,the normoxic group was housed in Xining city,Qinghai province(average altitude 2 200 m),while the hypoxic group was housed in a hypobaric oxygen chamber(simulated altitude 5 000 m).The two groups of pregnant rats were kept until day 21 of gestation.Blood samples were collected from the abdominal aorta and tail vein to determine fasting blood glucose(FBG).The serum insulin concentration of pregnant rats was measured by ELISA method.Placentas and embryos were collected for the comparisons of the weights of the placentas and fetal rats,as well as the length of fetal rats between the two groups.The placental structure of the pregnant rats in the two groups was observed under light microscopy after H-E staining,and the expression levels of GLUT1 mRNA and protein in placental tissues were detected by RT-qPCR and immunohistochemistry,respectively.The correlation between fetal rat weight and length with the FBG level and the relative expression of placental GLUT1 protein in pregnant rats was analyzed.Results:Compared with the normoxic group,the FBG level of pregnant rats in the hypoxic group was decreased and the serum insulin concentration was increased.The placental weight of the hypoxic group did not change,and the weight and length of fetal rats in the hypoxic group were decreased.Under the light microscopy,disordered arrangement of trophoblasts,widened intercellular space,and dilated capillaries with a large number of red blood cells were observed in the hypoxic group.The expression of GLUT1 mRNA in placental tissues of pregnant rats in the hypoxic group was up-regulated.The GLUT1-positive cells were distributed in trophoblasts and erythrocytes,mainly in the cell membrane,and the expression level of GLUT1 protein in the hypoxic group was increased.Pearson correlation analysis showed that fetal rat weight was positively correlated with FBG level in pregnant rats,while fetal rat length was negatively correlated with the relative expression of placental GLUT1 protein.Conclusion:The expression of GLUT1 in placental tissues of pregnant rats is up-regulated under plateau hypoxia,which subsequently affects the growth of fetal rats.关键词
高原低氧/妊娠大鼠/葡萄糖转运体1/空腹血糖/胎盘/胎鼠/发育Key words
plateau hypoxia/pregnant rat/glucose transporter 1/fasting blood glucose/placenta/fetal rat/development引用本文复制引用
陈文静,李建华,王雪妮,李丹,刘长钊..高原低氧下妊娠大鼠胎盘葡萄糖转运体1的表达及其对胎鼠生长发育的影响[J].解剖学杂志,2023,46(6):492-496,5.基金项目
青海省科技厅项目(2019-SF-134) （2019-SF-134）
