解剖学杂志2023，Vol.46Issue(6)：497-501,524,6.DOI:10.3969/j.issn.1001-1633.2023.06.006
金银花提取物联合奥曲肽对胰腺炎大鼠腺泡细胞损伤修复、免疫学指标及p38MAPK/ATF2水平的影响
Effects of Honeysuckle extract combined with Octreotide on acinar cell damage repair,immunological indicators,and p38MAPK/ATF2 levels in rats with pancreatitis
摘要
Abstract
Objective:To investigate the effects of Honeysuckle extract(HFE)combined with Octreotide on acinar cell injury repair,immune indices and p38MAPK/ATF2 levels in rats with severe pancreatitis.Methods:Forty rats were divided into healthy group,model group,HFE+Octreotide group,and Octreotide group.Severe pancreatitis models were established in all groups except the healthy group.The healthy group and model group were intraperitoneally injected with 1 mL/kg saline;the HFE+Octreotide group was injected with 120 mg/kg HFE and 10 μg/kg Octreotide via the tail vein;and the Octreotide group was injected with 10 μg/kg Octreotide via the tail vein.All injections were administered once daily for a total of four weeks.Immunoglobulins(IgA,IgG,IgM)were detected by enzyme-linked immunosorbent assay(ELISA).Immunohistochemistry was used to detect the p38 mitogen-activated protein kinase(p38MAPK)and activating transcription factor 2(ATF2)in rat pancreatic tissue.H-E staining was used to observe the pathological changes of pancreatic tissue.Protease and amylase activities were measured within acinar cells by using colorimetric method.Results:Compared with healthy group,the model group showed a decrease in serum levels of IgA,IgG and IgM,an increase in p38MAPK,ATF2,protease and amylase activities within acinar cells.Compared with the model group,the Octreotide group exhibited an increase in serum levels of IgA,IgG,IgM,a decrease in p38MAPK,ATF2,protease and amylase activities within acinar cells.Compared with the Octreotide group,the HFE+Octreotide group showed an increase in serum levels of IgA,IgG,IgM,and a decrease in p38MAPK,ATF2,protease and amylase activities within acinar cells.The model group exhibited more severe tissue damage and acinar cell injury.The Octreotide group showed improvements in lesion severity,cell quantity,and morphology,but structural damage was still present.In HFE+Octreotide group,cell degeneration and swelling were decreased,with gradual restoration of pancreatic tissue.Conclusion:Honeysuckle extract combined with Octreotide can treat severe pancreatitis in rats by repairing acinar cell damage and improving immune indices,the mechanism of which is related to the regulation of p38MAPK and ATF2 levels.关键词
胰腺炎/金银花提取物/奥曲肽/腺泡细胞/免疫/p38丝裂原活化蛋白激酶Key words
severe pancreatitis/Honeysuckle extract/Octreotide/acinar cells/immune/p38 mitogen-activated protein kinase引用本文复制引用
刘斐,姚杰,张丽骞..金银花提取物联合奥曲肽对胰腺炎大鼠腺泡细胞损伤修复、免疫学指标及p38MAPK/ATF2水平的影响[J].解剖学杂志,2023,46(6):497-501,524,6.基金项目
河北省医学科学研究基金(20200524) （20200524）
