解剖学杂志2023，Vol.46Issue(6)：502-507,513,7.DOI:10.3969/j.issn.1001-1633.2023.06.007
白藜芦醇通过减弱中性粒细胞迁移和浸润改善LPS诱导的急性肺损伤
Resveratrol improves LPS-induced acute lung injury by weakening migration and infiltration of neutrophils
摘要
Abstract
Objective:To explore the mechanism of Resveratrol(RSV)improving lipopolysaccharide(LPS)-induced acute lung injury(ALI)by weakened migration and infiltration of neutrophils.Methods:BALB/c mice were randomly divided into control group,RSV group,model group,and model+RSV group(LPS+RSV).In the model group and LPS+RSV group,ALI mice were induced by LPS,while mice in the RSV group and LPS+RSV group were intragastrically administered with 40 mg/kg of RSV for 7 d.After the last administration,bronchoalveolar lavage fluid(BALF)was collected to detect levels of white blood cells,neutrophils and proteins.The activity of myeloperoxidase(MPO)in lung tissues was evaluated.The pathological damage of lung tissues was observed by H-E staining.The expressions of inflammatory cytokines and chemokines in lung tissues and BALF were detected.Neutrophils were cultured in vitro and treated with 1 μg/mL of LPS and RSV,respectively.Neutrophils apoptosis and expression of chemokines receptors(CXCR2,Mac-1)were detected by flow cytometry.The level of reactive oxygen species(ROS)in cells was detected by dichlorodihydrofluorescein diacetate(DCFH-DA)probe to evaluate chemotaxis and migration ability of neutrophils.The phosphorylation level of nuclear factor(NF-κB p65)was detected by Western blotting.Results:Compared with the model group,the total count of cells,the count of neutrophils,expression level of proteins,expression levels of TNF-α,IL-6 and CXCL2 in BALF were decreased in the LPS+RSV group,and MPO activity in lung tissues,expression levels of TNF-α,IL-1β,IL-6,CXCL2 mRNA and protein were decreased.Compared with the control group,the apoptosis rate of neutrophils was decreased in the model group and the LPS+RSV group,but there was no significant difference in neutrophils apoptosis between the model group and the LPS+RSV group.Compared with the mode group,ROS activity in neutrophils was declined after 10 and 30 minutes of treatment in the LPS+RSV group,but there was no significant difference in ROS activity between the two groups at the other time points.Compared with the mode group,chemotaxis and migration ability of neutrophils,the number of neutrophils expressing CXCL2 and Mac-1 on the surface,expression of CXCL2 mRNA and protein,and p-NF-κB p65 were decreased in the LPS+RSV group.Conclusion:RSV can improve LPS-induced ALI,which may be related to the migration and infiltration of neutrophils inhibited by RSV.关键词
急性肺损伤/白藜芦醇/脂多糖/中性粒细胞/迁移/浸润Key words
acute lung injury/Resveratrol/lipopolysaccharide/neutrophil/migration/infiltration引用本文复制引用
刘新伟,李颖,李中霞,陈嘉宇,杨洋,林雪容..白藜芦醇通过减弱中性粒细胞迁移和浸润改善LPS诱导的急性肺损伤[J].解剖学杂志,2023,46(6):502-507,513,7.基金项目
2021年度河北省医学科学研究(20211689) （20211689）
