河南中医2024，Vol.44Issue(1)：62-70,9.DOI:10.16367/j.issn.1003-5028.2024.01.0013
基于网络药理学探讨柴胡-黄芩治疗青春期后痤疮的作用机制
Study on the Mechanism of Action of Chaihu-Huangqin in the Treatment of Post-Adolescent Acne
摘要
Abstract
Objective:To retrieve the main chemical active components of Chaihu(Radix Bupleuri)-Huangqin(Radix Scutellariae),and to explore the mechanism of action of Chaihu-Huangqin in the treatment of post-adolescent acne by using network pharmacology.Meth-ods:The active components of Chaihu and Huangqin were retrieved by using the pharmacological database and analysis platform of tradi-tional Chinese medicine system,and the corresponding targets were screened.The targets related to post-adolescent acne were retrieved by using the GeneCards database platform,and the common target Venn diagram was constructed.The potential target network of the ac-tive components was drawn by using Cytoscape 3.9.1 software,and the protein-protein interaction network was constructed by using the String database to obtain the number of adjacent nodes of the core gene of the protein-protein interaction(PPI)network,and then the topology analysis of the PPI network was performed by using Cytoscape 3.9.1 to obtain the core gene.The gene ontology(GO)func-tional annotation and Kyoto encyclopedia of genes and genomes(KEGG)pathway enrichment analysis were performed by using the newborn trust platform to predict the mechanism of action of Chaihu-Scalyxin in the treatment of post-adolescent acne.Results:The a-nalysis obtained 13 kinds of effective active components of Chaihu and 33 kinds of effective active components of Huangqin,involving 216 targets.A total of 1 899 targets of post-adolescent acne were identified,and 86 common target genes were obtained.By using PPI a-nalysis,tumor necrosis factor(TNF),tumor protein p53(TP53),RelA(NF-κB subtype),epidermal growth factor receptors(EG-FRs),interleukin(IL)-6,IL-1β 6 targets were identified as the core targets of Chaihu-Huangqin in the treatment of post-adolescent ac-ne.The enrichment analysis showed 3 526 GO entries and 230 KEGG signal pathways,mainly AGE-RAGE signal pathway,IL-17 signal pathway,TNF signal pathway,etc.Conclusion:Chaihu-Huangqin can treat post-adolescent acne through multiple targets and multiple pathways.While playing the role of antibacterial and anti-inflammatory,it can also reduce the formation of post-adolescent scars,provi-ding theoretical reference and research direction for the clinical application of Chaihu-Huangqin.关键词
青春期后痤疮/网络药理学/柴胡-黄芩/作用机制Key words
post-adolescent acne/network pharmacology/Chaihu(Radix Bupleuri)-Huangqin(Radix Scutellariae)/mechanism of ac-tion分类
医药卫生引用本文复制引用
李天举,刘永瑞,陈燕辉,丁治云,王维,张阳,付黎影,曹学辉,陈羽佳..基于网络药理学探讨柴胡-黄芩治疗青春期后痤疮的作用机制[J].河南中医,2024,44(1):62-70,9.基金项目
河南省医学科技攻关计划项目(LHGJ20210704) （LHGJ20210704）
