江西农业学报2023，Vol.35Issue(12)：1-8,8.DOI:10.19386/j.cnki.jxnyxb.2023.12.001
OsNramp5基因敲除对水稻产量的影响
Effects of OsNramp5 Gene Knockout on Rice Yield
摘要
Abstract
The plot yield comparison experiments were conducted in 3 different environments by random block design with multiple OsNramp5 gene knockout lines and their original cultivars as test materials,viz.Huazhan,Wushansimiao,Zhongzao 35 and Wufeng B.The results showed that although the OsNramp5 knockout materials showed superior cadmium reduction properties,almost all of them showed varying degrees of yield reduction(2.9%～25.6%)in different environments.Among the 42 yield comparison events between different OsNramp5 gene knockout strains and wild type controls,57.1%showed yield reduction rate more than 4%(statistically significant),28.6%showed a reduction of more than 5%(not statistically significant),11.9%showed a reduction of less than 4%(not significant),and 2.4%showed a slight increase(by 2.6%)in production(only 1 case;not significant).The yield decrease rate of the same OsNramp5 knockout lines was different in different environments.The yield variation degree of most OsNramp5 gene knockout strains was larger than that of the original varieties,and the fluctuation of yield decrease rate of the OsNramp5 knockout lines in different environments varied with cultivar backgrounds.In addition,there were cases that,different OsNramp5 knockout lines of the same variety background(there were differences in genomic background mutations during tissue culture between lines)and different sister lines of isolated offspring(from a common ancestor line)with the same knockout genotype,had significant differences in yield.The effect of OsNramp5 gene knockout on rice yield was influenced by cultivar type,somatic variation and environment,and the yield stability of most OsNramp5 knockout materials was impaired.Therefore,it is recommended to select suitable varieties for OsNramp5 gene knockout,and carefully select excellent strains or backcross after obtaining gene knockout strains,and carry out multi-year multi-point tests before application.关键词
水稻/低镉/OsNramp5/基因编辑/产量/稳产性Key words
Rice/Low cadmium/OsNramp5/Gene editing/Yield/Stability分类
农业科技引用本文复制引用
龙起樟,万建林,贺更清,苌兴超,黄永兰,王燕宁,唐秀英,王会民,芦明,徐斌..OsNramp5基因敲除对水稻产量的影响[J].江西农业学报,2023,35(12):1-8,8.基金项目
江西现代农业科研协同创新专项(JXXTCX201904-01) （JXXTCX201904-01）
江西省重大科技研发专项(20203ABC28W013) （20203ABC28W013）
江西省重点研发计划(20224BBF61030) （20224BBF61030）
江西省农业科学院博士启动基金项目(2012CBS002). （2012CBS002）
