江西农业学报2023，Vol.35Issue(12)：106-112,118,8.DOI:10.19386/j.cnki.jxnyxb.2023.12.015
六星瓢虫线粒体全基因组测序分析及系统发育研究
Mitochondrial Whole Genome Sequencing and Phylogenetic Analysis of Oenopia formosana
摘要
Abstract
The predatory predator ladybird Oenopia formosana within the genus Oenopia was taken as the research object,and the mitochondrial genome of O.formosana was determined.The mitochondrial genome characteristics of O.formosana were defined by comparative analysis,and the phylogenetic relationship of Coccinellidae was demonstrated by constructing phylogenetic trees.The results showed that:(1)The total length of mitochondrial genome of O.formosana was 17885 bp(GenBank entry number:OR804096),containing 37 genes,including 13 PCGs,2 rRNA genes,22 tRNA genes and a control region,was consistent with other published mitochondrial genomes of Coccinellidae,without rearrangement.(2)The AT content of the mitochondrial genome was 79.1%,and the top three codons used most frequently by protein-coding genes were UUA,AUU and UUU.(3)The maximum likelihood tree results based on the 13PCGs+2rRNAs gene sequence data set showed that the monophylety of each subfamily within Coccinellidae was supported.The phylogenetic relationship within the family is(Sticholotidinae +((Epilachninae+Scymninae)+ Coccinellinae)).(4)The newly sequenced O.formosana was most closely related to the ladybird Aiolocaria hexaspilota.Within Coccinellini,The phylogenetic relationship is((Anatis+(Calvia+Propylea))+((Hippodamia+Harmonia)+(Cycloneda+(Coccinella+(Oenopia+Aiolocaria))))).This study enriches the mitochondrial genome data of Coccinellidae and provides a reference for resolving the phylogenetic relationships of Coccinellidae.关键词
六星瓢虫/瓢虫科/线粒体基因组/系统发育Key words
Oenopia formosana/Coccinellidae/Mitochondria1genome/Phylogeny分类
生物科学引用本文复制引用
杜一民,欧阳智刚,卢占军..六星瓢虫线粒体全基因组测序分析及系统发育研究[J].江西农业学报,2023,35(12):106-112,118,8.基金项目
江西省重大科技研发专项(20194ABC28007) （20194ABC28007）
江西省现代农业产业技术体系建设专项(JXARS-07). （JXARS-07）
