口腔颌面外科杂志2024，Vol.34Issue(1)：22-28,7.DOI:10.12439/kqhm.1005-4979.2024.01.003
基于人诱导多能干细胞的组织工程化软骨再生
Tissue-engineered cartilage regeneration based on human induced pluripotent stem cells
摘要
Abstract
Objective:To establish a stable and efficient culture approach for guiding human induced pluripotent stem cells(hiPSCs)differentiation towards chondrogenic mesodermal lineage,and preliminarily explore the possibility of hiPSCs-derived cartilaginous pellets for articular cartilage regeneration.Methods:By simulating the development process in vivo,hiPSCs were gradually induced to differentiate into chondrogenic mesodermal lineage and mature cartilage using three dimensions(3D)suspension culture system.Real-time quantitative polymerase chain reaction(RT-qPCR),Western blotting,immunofluorescence and immunohistochemistry were used to investigate the dynamic expression change of pluripotent,mesodermal and chondrongenic related genes and proteins during the whole culture procedure.Finally,subcutaneous model of nude mice was used to investigate the stability of chondrogenic phenotypes and tumorigenicity of hiPSCs derived-cartilaginous pellets.Results:By simulating the development process in vivo,hiPSCs were gradually induced to differentiate into chondrogenic mesodermal lineage,and finally differentiated into mature cartilage tissues.Conclusion:In this study,a stable and efficient culture approach for inducing hiPSCs differentiation towards chondrogenic mesoderm was successfully established,which provide a research platform for investigation of hiPSCs-mediated cartilage development and regeneration.关键词
人类诱导多能干细胞/中胚层/悬浮培养/软骨球Key words
human induced pluripotent stem cells/mesoderm/suspension culture/cartilaginous pellets分类
医药卫生引用本文复制引用
李阳阳,张茂林,邹多宏,张志愿..基于人诱导多能干细胞的组织工程化软骨再生[J].口腔颌面外科杂志,2024,34(1):22-28,7.基金项目
国家自然科学基金(32171347) （32171347）
2022年度上海市卫生健康委员会学科带头人(2022XD038) （2022XD038）
上海交通大学医学院附属第九人民医院交叉基金(JYJC202012) （JYJC202012）
