摘要
Abstract
Objective:To investigate the effect of curcumin-mediated exosomes of bone marrow mesenchymal stem cells(BMSCs)on proliferation and metastasis of ACC-M cells and its mechanism.Methods:After being co-cultured with 7.5 µmmol/L curcumin and BMSCs for 72 h,exosomes were isolated and identified,and co-cultured with ACC-M cells.ACC-M cells were divided into control group(normal culture),BMSC-Exo group(no curcumin-treated BMSCs exosomes co-cultured with ACC-M cells),Cur-BMSC-Exo group(curcumin-treated BMSCs exosomes co-cultured with ACC-M cells)and Cur group(7.5 µmmol/L curcumin treated cells).Western blotting was used to detect the expression of tumor susceptibility gene 101(TSG-101),CD63,CD9 and recombinant human calnexin(CANX)proteins in exosome and the expression of E-cadherin,N-cadherin,vimentin,transforming growth factor-β1(TGF-β1)and p-ERK proteins in ACC-M cells after 48 h of culture.The survival rate of ACC-M cells was determined by cell counting kit-8(CCK-8)assay.The migration and invasion of ACC-M cells were detected by transwell assay.The relative expression levels of TGF-β1 and ERK mRNA in ACC-M cells were detected by real-time quantitative polymerase chain reaction(RT-qPCR)assay.Results:Compared with control group and BMSC-Exo group,the cell viability,cell migration and invasion number,and the expression levels of N-cadherin,vimentin,TGF-β1 and ERK were significantly decreased in Cur-BMSC-Exo and Cur groups(P<0.05),while E-cadherin protein level was significantly increased(P<0.05);compared with the Cur-BMSC-Exo group,the cell viability,cell migration and invasion numbers,and the expression levels of N-cadherin,vimentin,TGF-β1 and ERK were significantly increased in the Cur group(P<0.05),while E-cadherin protein level was significantly decreased(P<0.05).Conclusion:BMSCs exosomes can act as curcumin carrier to inhibit proliferation and metastasis of ACC-M cells and regulate TGF-β1/ERK signaling pathway.关键词
姜黄素/骨髓间充质干细胞/外泌体/ACC-M/增殖Key words
curcumin/bone marrow mesenchymal stem cells/exosome/ACC-M/proliferation分类
医药卫生
