棉花学报2023，Vol.35Issue(6)：447-458,12.DOI:10.11963/cs20230039
类钙调磷酸酶B亚基蛋白GhCBL3-A01调控棉花黄萎病抗性的功能分析
Functional analysis of cotton calcineurin B-like protein GhCBL3-A01 in regulating the resistance to Verticillium wilt
摘要
Abstract
[Objective]This research aims to investigate the role of calcium B-like protein(CBL)gene GhCBL3-A01 in resistance to Verticillium wilt in cotton,so as to provide genetic resource for cotton disease resistance breeding.[Methods]The homologous sequences of GhCBL3-A01 gene were obtained from cotton genomic database for bioinformatic analysis.The relative expression levels of GhCBL3-A01 under Verticillium dahliae,methyl jasminate(MeJA)and H2O2 treatments were detected by quantitative real-time polymerase chain reaction(qRT-PCR).The function of GhCBL3-A01 in cotton resistance to Verticillium wilt was analysed by virus-induced gene silencing(VIGS)technique.The functional mechanisms of GhCBL3-A01 were analyzed by detecting the accumulation of reactive oxygen species(ROS)and the expression levels of related genes.[Results]GhCBL3-A01 and its three homologous proteins in upland cotton all contain three typical EF-hand domains of the CBL family.The expression level of GhCBL3-A01 was significantly increased under the treatment of Verticillium dahliae,MeJA,and H2O2.Silencing GhCBL3-A01 by VIGS resulted in a significant decrease in rate of diseased plants and disease index,a reduction in the browning degree of vascular bundles,and a decline in the number of hyphae in stem segments,which enhanced the resistance of cotton plants to Verticillium wilt.The expression levels of disease resistance-related genes(PR1,NPR1,PR4,and PDF1.2)and jasmonic acid(JA)signaling pathway genes(AOS1,OPR3,MYC2,and LOX2)were increased,and ROS accumulation was enriched in GhCBL3-A01 silenced cotton plants.[Conclusion]GhCBL3-A01 negatively regulates the resistance of cotton to Verticillium wilt by regulating the expression levels of defense-related genes and JA signaling pathway genes as well as the accumulation of ROS,which is a candidate gene for improving the resistance of cotton to Verticillium wilt.关键词
棉花/黄萎病/类钙调磷酸酶B亚基蛋白/GhCBL3-A01/抗病基因/病毒诱导的基因沉默Key words
cotton/Verticillium wilt/calcineurin B-like protein(CBL)/GhCBL3-A01/resistance gene/virus-induced gene silencing引用本文复制引用
高升旗,邵武奎,赵准,邵盘霞,胡文冉,黄全生..类钙调磷酸酶B亚基蛋白GhCBL3-A01调控棉花黄萎病抗性的功能分析[J].棉花学报,2023,35(6):447-458,12.基金项目
国家自然科学基金——新疆联合基金(U1703114) （U1703114）
中央引导地方科技发展专项资金项目(ZYYD2022B07) （ZYYD2022B07）
