棉花学报2023，Vol.35Issue(6)：497-508,12.DOI:10.11963/cs20230024
两个棉蚜胰岛素受体生物信息学分析及其在三种有翅型棉蚜中的表达模式比较
Bioinformatics analysis of two insulin receptors in Aphis gossypii and comparison of their expression patterns in three winged phenotypes
摘要
Abstract
[Objective]This study aims to clarify the sequence structure and expression pattern of two insulin receptor genes in three kinds of winged aphids of Aphis gossypii.[Methods]Based on the whole genome data of A.gossypii,amino acid sequence analysis of two insulin receptor genes(insulin receptor 1,AgInR1;insulin receptor 2,AgInR2)was identified,and phylogenetic analysis of insect InR1 from 17 insects and InR2 from 14 insect was performed.The protein conserved structural domains of insulin receptors were analyzed and motif analysis was conducted using online software.Quantitative real-time polymerase chain reaction(qRT-PCR)was used to analyze the expression patterns of AgInR1 and AgInR2in wingless female and three kinds of winged aphids at different developmental stages.[Results]The physicochemical analysis showed that AgInR1 and AgInR2 were hydrophilic transmembrane proteins.Phylogenetic analysis showed that InR1 and InR2 of the analyzed insects were clustered into two distinctive groups.The InR1 and InR2 of Hemiptera Aphididae were clustered together with very high bootstrap values,showed close affinity.Conserved domains and motif analyses showed that conserved domain arrangement of InR1 and InR2 from A.gossypii were relatively similar.Compared with AgInR1,AgInR2 lacked one FN3 domain.The results of qRT-PCR showed that the expression level of AgInR1 in the 4th instar and adult stages of the three winged aphids,namely,parthenogenetic wing female,gynoparae,and male was significantly higher than that of parthenogenetic wingless female at the same instar.Meanwhile the expression pattern of AgInR2 in the three kinds of winged aphids was different from that of AgInR1.[Conclusion]AgInR1 might involve in regulating the wing development process in the 4th instar and adult stages of three winged aphids.AgInR2 had a different expression pattern from AgInR1 and might have different biological functions.The study provides theoretical support for further investigation of the role of insulin signaling pathway in wing differentiation of A.gossypii.关键词
棉蚜/翅多态性/胰岛素受体/表达模式/翅型分化Key words
Aphis gossypii/wing polymorphism/insulin receptor/expression patterns/wing dimorphism引用本文复制引用
王柳玉,王丽,朱香镇,张开心,李东阳,姬继超,崔金杰,马伟华..两个棉蚜胰岛素受体生物信息学分析及其在三种有翅型棉蚜中的表达模式比较[J].棉花学报,2023,35(6):497-508,12.基金项目
国家自然科学基金(32102214) （32102214）
