棉花学报2023，Vol.35Issue(6)：509-518,10.DOI:10.11963/cs20220050
枯草芽孢杆菌NCD-2与化学杀菌剂复配对棉花黄萎病的防治效果
Control effects of combination of Bacillus subtilis NCD-2 and chemical fungicides on cotton Verticillium wilt
摘要
Abstract
[Objective]This research aims to study the effects of the combination of Bacillus subtilis NCD-2 and chemical fungicides on the control of cotton Verticillium wilt,so as to provide a theoretical basis for the control of cotton Verticillium wilt.[Methods]The virulent Verticillium dahliae strain Vd080,a pathogen causing cotton Verticillium wilt was used as the object,and the virulence of 5 fungicides(tebuconazole,fluazinam,prochloraz,mancozeb and chlorothalonil)were determined by growth rate method.The toxicity of NCD-2 to Vd080 was determined by dual-culture method.The fungicides with better compatibility with NCD-2 were selected,and the volume ratio of fungicides and NCD-2 was further determined by Horsfall method.The control effects on cotton Verticillium wilt of different compound treatments were evaluated in the indoor pot experiment.[Results]Among the 5 fungicides,prochloraz had the best inhibitory effect on Vd080,and its median effective concentration(EC50)was 0.027 mg·L-1,followed by tebuconazole and fluazinam.The EC50 of NCD-2 to Vd080 is 2.56×107 mL-1(cell concentration).The compatibility of 0.4 mg·L-1 fluazinam with 2.56×107 mL-1 NCD-2 was best,reaching 79.19%.The compatibility of 2.0 mg·L-1 fluazinam and 0.02 mg·L-1 prochloraz with 2.56×107 mL-1 NCD-2 were 71.29%and 63.20%,respectively.The synergistic effect of 2.56×107 mL-1 NCD-2 was the strongest when mixed with 0.4 mg·L-1 fluazinam,2.0 mg·L-1 fluazinam,and 0.02 mg·L-1 prochloraz at the volume ratios of 3∶7,9∶1 and 1∶1,respectively,and the inhibitory effect on Vd080 was the best.The indoor pot experiment showed that when 2.56×107 mL-1 Bacillus subtilis NCD-2 and 0.02 mg·L-1 prochloraz were mixed at the volume ratio of 1∶1,the disease index of cotton Verticillium wilt was the lowest,the control effect was the best(82.81%),and the synergistic effect was good(synergistic coefficient was 23.46).[Conclusion]The combination of 2.56×107 mL-1 Bacillus subtilis NCD-2 and 0.02 mg·L-1 prochloraz at the volume ratio of 1∶1 has a good control effect on cotton Verticillium wilt,and good synergistic effect.关键词
棉花/黄萎病/枯草芽孢杆菌/杀菌剂/协同防治Key words
cotton/Verticillium wilt/Bacillus subtilis/fungicide/synergistic control引用本文复制引用
郑德有,左东云,吕丽敏,王巧莲,程海亮,顾爱星,宋国立..枯草芽孢杆菌NCD-2与化学杀菌剂复配对棉花黄萎病的防治效果[J].棉花学报,2023,35(6):509-518,10.基金项目
国家自然科学基金(31621005) （31621005）
中国农业科学院科技创新工程(CAAS-ASTIP-CCRI) （CAAS-ASTIP-CCRI）
