油茶IDD基因家族鉴定与生物信息学分析OA北大核心CSTPCD

Camellia oleifera is a unique oil plant of Theaceae,which has important ornamental value and economic value.With the intensive planting of Camellia,many diseases and pests occur.In addition,the delayed breeding of im-proved varieties seriously restricts the development of the Camellia industry.INDETERMINATE DOMAIN(IDD)fam-ily is a kind of conservative transcription factors in higher plants,which regulate the basic immune response of plants by mediating plant endogenous hormones.In order to identify the members of IDD gene family in C.oleifera,29 members of CoIDD gene family were identified through the conserved domain of IDD gene family with reference to the whole genome information of C.oleifera,and further clarified the structure,physical and chemical properties,phylogenetic relationship,and sub cell localization of each gene.The CoIDD4 gene homologous to AtIDD4/5/6 was cloned by RT-PCR,and its physicochemical properties,expression mode and nuclear localization signal were clarified.At the same time,the types of cis acting elements in its promoter region were analyzed.The results showed that 29 CoIDD gene family members were divided into 4 subgroups,with average hydrophilicity coefficients less than 0,belonging to hydrophilic proteins,and subcellular localization was located in the nucleus;The full-length ORF frame cloned into CoIDD4 gene contained 1620 bp base and encoded 539 amino acids,which were mainly expressed in leaf and flower organs;The cis acting elements in the promoter region of the gene were mainly involved in light response and plant hormone response elements.In conclusion,this study identified 29 IDD gene family members in C.oleifera,among which CoIDD4 gene was closely related to AtIDD4/5/6.It is speculated that CoIDD4 gene may be involved in the proc-ess of hormone signal transduction and basic immune response regulation of C.oleifera,which would provide a theo-retical basis for the functional analysis of IDD transcription factors in C.oleifera and provide a reference basis for the selection and molecular breeding of superior resistant varieties of C.oleifera.