农药学学报2024，Vol.26Issue(1)：149-159,11.DOI:10.16801/j.issn.1008-7303.2023.0102
氯氟醚菊酯对斑马鱼胚胎及仔鱼心脏发育的毒性及氧化损伤作用
Toxicity and oxidative damage of meperfluthrin on cardiac development of zebrafish embryos and larvae
摘要
Abstract
Meperfluthrin is one of the top-selling sanitary insecticide species in the domestic market,drawing widespread attention due to its environmental safety risk.In this paper,zebrafish was used as an experimental model to assess the cardiac developmental toxicity and oxidative damage caused by acute exposure to meperfluthrin in embryos and larvae.The acute toxicity assessment revealed that meperfluthrin is highly toxic to zebrafish embryos with a 72 h-LC50 of 0.756 μg/mL.After exposure to different doses of meperfluthrin(0,0.2,0.4,0.8 μg/mL)for 72 h,the larvae from 0.4 and 0.8 μg/mL dose-treated groups showed deformities,including spine and tail curvature,pericardium and yolk sac edema,significant reduction of heart rate and hatchability,and incomplete embryonic development(shorter body length,smaller head,and eye area).Moreover,meperfluthrin of 0.2,0.4,and 0.8 μg/mL induced atrial and ventricular differentiation in larvae,and down-regulated the expression of cardiac function-related genes myl7,myh6,and vmhc.It also increased the content of malondialdehyde(MDA),as well as decreased the activities of antioxidant enzymes such as superoxide dismutase(SOD),catalase(CAT),and glutathione peroxidase(GSH-Px),resulting in the accumulation of reactive oxygen species(ROS)in the heart of larvae.The results of this paper indicate that meperfluthrin exposure has signifi-cant toxicity to the cardiac development of zebrafish embryos and is associated with oxidative damage.关键词
卫生杀虫剂/氯氟醚菊酯/环境风险/斑马鱼/发育毒性/氧化应激Key words
hygienic pesticides/meperfluthrin/environmental risk/zebrafish/developmental toxicity/oxidative stress分类
农业科技引用本文复制引用
李瑞瑞,周宁,吴梦琪,徐文平,陶黎明,张阳..氯氟醚菊酯对斑马鱼胚胎及仔鱼心脏发育的毒性及氧化损伤作用[J].农药学学报,2024,26(1):149-159,11.基金项目
国家重点研发计划项目(2022YFD1700501). Supported by National Key Research and Development Program of China(No.2022YFD1700501). （2022YFD1700501）
