肿瘤预防与治疗2024，Vol.37Issue(2)：101-109,9.DOI:10.3969/j.issn.1674-0904.2024.02.002
丙泊酚改善缺氧诱导的宫颈癌细胞增殖、G2/M期阻滞和辐射抗性
Propofol Improved the Proliferation,G2/M Phase Arrest and Radiation Resistance of Cervical Cancer Cells Induced by Cobalt Chloride
摘要
Abstract
Objective:To investigate the effect of propofol on the proliferation,G2/M phase arrest and radiation resistance induced cobalt chloride(CoCl2)in on cervical cancer Hela cells and its relationship with HIF-1α and VEGF.Methods:CoCl2 was employed to construct a hypoxia model in Hela cells.The cell proliferative activity,cell cycle,cell radiation sensitivity,the relative mRNA and protein expression levels of HIF-1α and VEGF were detected by crystal violet staining,flow cytometry,cloning formation experiments,real-time quantitative PCR,western blotting and enzyme-linked immunosorbent assays,respectively.Results:Propofol inhibited the proliferation of Hela cells induced by CoCl2 in a dose-dependent manner.Compared with the control group,CoCl2 treatment induced a significant increase in the proportion of cells in the G0/G1/S phase and a significant decrease in the proportion of cells in the G2/M phase(P<0.001),which were significantly reversed after propofol treatment(P<0.001).In CoCl2-treated Hela cells,propofol reduced Dq,SF2,and D0 values after radiation at 0,1,2,and 4 Gy dose,and restored the down-regulation of transcriptional and translational expression levels of HIF-1 α and VEGF induced by CoCl2 treatment(P<0.001).Conclusion:Propofol might reverse anoxia-induced proliferation,G2/M phase arrest and radiation resistance by downregulating the expression of HIF-1α and VEGF in Hela cells.关键词
宫颈癌/丙泊酚/氯化钴/辐射抗性/HIF-1 α/VEGFKey words
Cervical cancer/Propofol/Cobalt chloride/Radiation resistance/HIF-1 α/VEGF分类
医药卫生引用本文复制引用
毛远舟,郭巧姗,黄建鸣,邹江,陈一丁,张可贤,舒进军..丙泊酚改善缺氧诱导的宫颈癌细胞增殖、G2/M期阻滞和辐射抗性[J].肿瘤预防与治疗,2024,37(2):101-109,9.基金项目
This study was supported by grants from Science and Technology Department of Sichuan Province(No.2020YFS0419),Sichuan Medical Association(No.S19019)and Sichuan Cancer Hospital(No.YB2016006). 四川省科技厅重点研发项目(编号:2020 YFS0419) （No.2020YFS0419）
四川省医学会科研课题(编号:S19019)四川省肿瘤医院麻醉学重点学科基金(编号:YB2016006) （编号:S19019）
