肿瘤预防与治疗2024，Vol.37Issue(2)：110-120,11.DOI:10.3969/j.issn.1674-0904.2024.02.003
过氧化物还原酶4在胃癌中的表达及其意义的生物信息学研究
Expression and Significance of Peroxiredoxin 4 in Gastric Cancer:A Bio-informatics Study
摘要
Abstract
Objective:To explore the expression and significance of PRDX4 in gastric cancer(GC)through bioinformatics,and to detect the expression of PRDX4 in tumor tissues from 63 GC patients with immunohistochemistry.Methods:The ex-pression data of PRDX4 in normal gastric tissues and primary gastric cancer tissues were obtained from Ualcan database,the co-expression genes of PRDX4 were searched on LinkedOmics,and the biological processes and functions of these genes were determined by GO enrichment analysis and KEGG pathway analysis.The interaction network of proteins encoded by related genes was analyzed using STRING database.Finally,GEPIA database was used to analyze the relationship between PRDX4 expression and prognosis of gastric cancer patients.A total of 63 GC patients treated in the First Affiliated Hospital of Chengdu Medical College from January 2022 to October 2022 were included.Immunohistochemical staining of GC tumor tissues and surrounding normal tissues was performed by MaxVision method and the results were observed.Results:Ualcan database showed that PRDX4 was highly expressed in GC tissues compared with surrounding normal gastric tissues,but no correlation was found between PRDX4 and sex,degree of tumor differentiation,tumor size and lymph node metastasis.Meanwhile,PRDX4 co-expression gene was closely related to molecular bio-logical processes such as biological regulation,metabolic process and stimulus response,and was significantly related to ErbB,Hip-po,Hedgehog and other signaling pathways and various cancer pathways.Several GC-related gene encoded proteins interacted with PRDX4 encoded proteins.Immunohistochemical and western blot analysis showed that PRDX4 was highly expressed in GC tissues.Conclusion:PRDX4 is highly expressed in GC tissues and is closely related to the ongenesis and development of GC,which is expect-ed to be a marker for GC screening.关键词
过氧化物还原酶4/胃癌/生物标记/生物信息学Key words
Peroxiredoxin 4/Gastric cancer/Biomarkers/Bioinformatics分类
医药卫生引用本文复制引用
张博凯,王子雨,李佳芪,宗咏花,王骁,胡晓松,李娟..过氧化物还原酶4在胃癌中的表达及其意义的生物信息学研究[J].肿瘤预防与治疗,2024,37(2):110-120,11.基金项目
This study was supported by China National University Student Innovation&Entrepreneurship Development Program(No.S202013705007),and by grants from Chengdu Medical College(No.2020dy178). 国家级大学生创新创业项目(编号:S2020 13705007) （No.S202013705007）
成都医学院调研类大学生创新创业项目(编号:2020dy178) （编号:2020dy178）
