妇儿健康导刊2024，Vol.3Issue(2)：190-194,封3,6.
花旗松素对人卵巢癌的作用机制
Mechanism of action of taxifolin on human ovarian cancer
孙同同 1张栋栋 2张堃 2徐蕾2
作者信息
- 1. 山东第一医科大学研究生院,山东济南 250000
- 2. 山东省淄博市妇幼保健院妇科,山东淄博 255000
- 折叠
摘要
Abstract
Objective To explore the mechanism of action of taxifolin in the treatment of ovarian cancer based on network pharmacology and in vitro cell experiments.Methods Literature and databases were searched to collect the targets of action of taxifolin,predict the targets in SuperPred database,download disease genes and find the intersection between drugs and disease targets,perform protein-protein interaction analysis,obtain the core targets,and perform enrichment analysis and molecular docking.Human ovarian cancer SKOV3 cells were cultured in vitro,and the blank control group and the taxifolin treated group(10,30 µg/ml)were set up.The cell inhibition rates and the expression of phosphorylated serine/threonine kinase proteins(p-AKT)and serine/threonine kinase proteins(AKT)of each group were compared.Results There were 79 potential targets and 12 core targets for the action of taxifolin on ovarian cancer.The enrichment analysis results indicated abnormal expression of target proteins in cancer cells,which affected enzyme and protein binding functions by regulating metabolic and other biological processes.KEGG was mainly enriched in the PI3K/AKT pathway,and molecular docking suggested that taxifolin had good binding activity with various targets.In vitro experiments confirmed that taxifolin could inhibit the activity of ovarian cancer cells.Western blot results showed that taxifolin could reduce the expression of p-AKT/AKT protein,thereby exerting anti-tumor effects.Conclusion Taxifolin can inhibit the proliferation of ovarian cancer cells through the PI3K/AKT pathway.关键词
花旗松素/卵巢癌/网络药理学/作用机制Key words
Taxifolin/Ovarian cancer/Network pharmacology/Mechanism of action分类
医药卫生引用本文复制引用
孙同同,张栋栋,张堃,徐蕾..花旗松素对人卵巢癌的作用机制[J].妇儿健康导刊,2024,3(2):190-194,封3,6.
