生态科学2023，Vol.42Issue(6)：116-126,11.DOI:10.14108/j.cnki.1008-8873.2023.06.012
模拟垂直流人工湿地不同木本植物根际细菌群落特征
Traits of bacterial communities in the rhizospheres of different woody plants in simulated vertical flow constructed wetland
摘要
Abstract
The study was conducted to profile the community structure of rhizospheric microbes and investigate the key functional groups of seven woody plants including Adina rubella,Salix rosthornii,Callicarpa dichotoma,Nerium oleander,Hibiscus mutabilis,Ligustrum obtusifoliumand Ligustrum lucidum,by using high-throughput sequencing technology and the constructed wetland microcosmic reactor as the experimental model.The results showed that the diversity and evenness of bacterial community in the rhizospheres of Adina rubella and Ligustrum obtusifolium were higher than those of the other five species,and their root systems could better accumulate microorganisms.At the phylum level,Proteobacteria(34.7%)and Firmicutes(31.7%)were the phyla with the highest average relative abundance among 7 different woody plants,followed by Chloroflexus(7.4%),Actinomycete(6.8%)and Cyanobacteria(6.5%);at the genus level,Lactobacillus,Pediococcus and Geobacter were the common dominant genera of woody plants,with relative abundance of 11.5%,6.5%,and 2.5%,respectively.Principal component analysis showed that there were significant differences in the colony composition of Ligustrum lucidum,Callicarpa dichotoma and Nerium oleander.Lactobacillus,Pediococcus and Lactococcus were the key genera that caused colony differences among all woody plant samples.KEGG metabolic pathway analysis showed that the relative abundance of nitrobacteria in the rhizospheres of different woody plants was generally low,ranging from 0.11%to 1.06%;Ligustrum obtusifolium had the strongest ability to enrich denitrifying bacteria,with relative abundance of 3.51%-10.40%.Therefore,it can be concluded that anaerobic conditions promoted the process of microbial denitrification under the condition of vertical flow constructed wetland.Meanwhile,denitrifying bacteria could be enriched in the rhizospheres of woody plants.All the tested woody plants could guarantee the denitrifying ability of the constructed wetland system while adapting to the stress of anaerobic conditions.关键词
垂直流人工湿地/木本植物/根际菌群/16S rRNA细菌高通量测序Key words
vertical flow constructed wetland/woody plant/rhizosphere flora/16S rRNA bacterial high-throughput sequencing分类
生物科学引用本文复制引用
王宇阳,岳春雷,谷雅馨,李贺鹏,王珺,房瑶瑶..模拟垂直流人工湿地不同木本植物根际细菌群落特征[J].生态科学,2023,42(6):116-126,11.基金项目
浙江省"领雁"研发攻关计划项目(2022C02038) （2022C02038）
浙江省院所专项(2019F1065-1) （2019F1065-1）
