医学新知2024，Vol.34Issue(2)：121-128,8.DOI:10.12173/j.issn.1004-5511.202401008
前列腺癌中PI3K/AKT通路调控PHF19基因表达的机制研究
Mechanism research of PI3K/AKT pathway regulating PHF19 gene expression in prostate cancer
摘要
Abstract
Objective To investigate the regulatory effect of the PI3K/AKT pathway on PHF19 gene expression in prostate cancer.Methods PC-3 cells were treated with the PI3K/AKT pathway inhibitor LY294002 or the activator EGF.Subsequently,the expression of TP63,ΔNp63 and PHF19 mRNA was detected using quantitative reverse transcription PCR(qRT-PCR).Potential transcription factors involved in PHF19 gene regulation were predicted using ChIPBase and JASPAR databases.The correlation between mRNA expressions of TP53,TP63 and PHF19 genes was analyzed through the GEPIA 2 database.Chromatin immunoprecipitation followed by quantitative PCR(ChIP-qPCR)was employed to assess the binding of ΔNP63 protein to the PHF19 gene promoter.Results LY294002 significantly reduced PHF19 mRNA expression(compared with different time treatment groups,P＜0.05),while EGF notably enhanced the PHF19 mRNA expression(compared with different time treatment groups,P＜0.05).TP63 mRNA was positively correlated with PHF19 mRNA expression in prostate cancer tissues(r=0.43,P＜0.05).ΔNp63 could bind to the promoter region of the PHF19 gene.LY294002 also significantly reduced the expression of both TP63 and ΔNp63 mRNA(compared with different time treatment groups,P＜0.05).Conclusion The PI3K/AKT pathway may enhance PHF19 gene expression via the transcription factor TP63.关键词
PI3K/AKT/TP63/PHF19/前列腺癌Key words
PI3K/AKT/TP63/PHF19/Prostate cancer引用本文复制引用
明道靖,郭梦梦,张晋辉,刘梦洋,史明慧,刘姝言,袁帅,曾宪涛..前列腺癌中PI3K/AKT通路调控PHF19基因表达的机制研究[J].医学新知,2024,34(2):121-128,8.基金项目
国家自然科学基金面上项目(82373411) （82373411）
湖北省自然科学基金面上项目(2023AFB742) （2023AFB742）
