中国动物检疫2024，Vol.41Issue(2)：58-64,7.DOI:10.3969/j.issn.1005-944X.2024.02.011
微流控芯片环介导等温扩增技术检测3种猪圆环病毒
Microfluidic Chip Loop-mediated Isothermal Amplification for Detecting Three Porcine Circoviruses
摘要
Abstract
In order to rapidly distinguish three types of porcine circoviruses(PCV2,PCV3 and PCV4)at field level,clinical positive samples infected with the three circoviruses were extracted for nucleic acids for microfluidic chip loop-mediated isothermal amplification,the results of which were synchronously compared with those of three commercial fluorescent probe kits in the aspects of sensitivity,specificity and repeatability.The results indicated that the microfluidic chip loop-mediated isothermal amplification method was highly sensitive in joint-detection of the three viruses,with the sensitivity no lower than fluorescent quantification PCR within 30 minutes,failed to crossly react with clinical positive samples infected with African swine fever virus(ASFV),classical swine fever virus(CSFV),porcine reproductive and respiratory syndrome virus(PRRSV),porcine pseudorabies virus(PRV)and porcine parvovirus(PPV),indicating good specificity;and all the coefficients of variation(CVs)of the repeated Ct values after detection of the three viruses were below 2%,showing good stability.In conclusion,the microfluidic chip method for joint-detection of the three viruses was characterized with the advantages of good specificity,high sensitivity,strong repeatability,rapid detection and low requirements for environment,and appropriate for rapid on-site detection of porcine circoviruses on swine farms and relevant premises.The established method could be used as a strong tool for rapid on-site detection of relevant pig pathogens.关键词
猪圆环病毒/微流控芯片/环介导恒温扩增/快速检测/核酸检测Key words
porcine circovirus/microfluidic chip/loop-mediated isothermal amplification/rapid detection/nucleic acid test分类
农业科技引用本文复制引用
石艳萍,邓飞,周丽媛,李丽,邵靓,陈斌,张孟思,邱明双,陈弟诗..微流控芯片环介导等温扩增技术检测3种猪圆环病毒[J].中国动物检疫,2024,41(2):58-64,7.基金项目
四川省科技计划重点研发项目(2023YFN0021) （2023YFN0021）
