中国动物检疫2024，Vol.41Issue(2)：80-85,6.DOI:10.3969/j.issn.1005-944X.2024.02.014
3种方法检测禽网状内皮组织增殖病毒人工感染鸡胚孵化雏鸡的带毒比较
Comparison of Three Methods for Detecting the Viral Load in Chicken-embryo Chicks Artificially Infected with REV
摘要
Abstract
In order to compare the accuracy of various methods for detecting avian reticuloendotheliosis virus(REV)in blood and fecal samples and thus to provide a reference for the development of purification procedures for avian reticuloendotheliosis(RE),a model for REV vertical transmission to chicks was constructed using chickembryoyolksac inoculation.Blood and meconium samples from 1-day-old chicks,blood and cloacal swabs from 7 to 70-day-old chicks(every other week)were detected by RT-PCR,fluorescence quantitative RT-PCR and virus isolation method using DF-1 cells,respectively,and the results from which were compared.The results revealed that,for the plasma from 1-day-old SPF chicks,fluorescence quantitative RT-PCR had the highest sensitivity and positive detection rate of 100%,followed by virus isolation mehtod with the positive detection rate of 96%(24/25),and the last one was RT-PCR;for the meconium samples of 1-day-old SPF chicks,fluorescence quantitative RT-PCR and virus isolation shared the same sensitivity with the positive detection rate of 80%(20/25),and RT-PCR had the lowest detection rate of 28%(7/25).For the blood and cloacal swabs from 7 to 70-day-old chicks(every other week),the detection rates by the three methods were generally similar to those of 1-day-old chicks,that was,fluorescence quantitative RT-PCR,virus isolation and RT-PCR in successive order from high to low.In conclusion,the positive detection rate of REV in meconium/cloacal swabs was generally lower than that in blood using the same method for detecting samples within the same period.The methods for detecting the viral load of chicken-embryo chicks artificially infected with REV were preliminarily compared in the study,laying a foundation for future implementation of REV purification.关键词
禽网状内皮组织增殖病毒/病毒分离/RT-PCR/荧光定量RT-PCRKey words
REV/virus isolation/RT-PCR/fluorescence quantitative RT-PCR分类
农业科技引用本文复制引用
徐凤霞,孙万里,张亚文,常爽,王一新,赵鹏..3种方法检测禽网状内皮组织增殖病毒人工感染鸡胚孵化雏鸡的带毒比较[J].中国动物检疫,2024,41(2):80-85,6.基金项目
山东省重点研发计划(乡村振兴科技创新提振行动计划)项目(2022TZXD0019) （乡村振兴科技创新提振行动计划）
