中国动物检疫2024，Vol.41Issue(2)：86-91,6.DOI:10.3969/j.issn.1005-944X.2024.02.015
纳米孔测序技术对一份禽流感病毒阳性样品的分子溯源
Molecular Tracing of a Positive Sample with Avian Influenza Virus by Nanopore Sequencing
摘要
Abstract
H5 subtype avian influenza(AI)is a zoonotic disease posing a serious threat to human and animal health.It is important to disclose the molecular characteristics of avian influenza virus(AIV)through targeted whole genome sequencing and phylogenetic analysis.In the study,the whole genome of AIV in one positive swab sample was sequenced by the third-generation of targeted nanopore technology.The sequence identification results showed that H5N5 subtype AIV was identified,its sequence coverage was 96.44%,only leaving about 480 bp sequence of PB1 gene;HA protein cleavage site contained 5 consecutive alkaline amino acids,with the molecular characteristics of highly pathogenic avian influenza virus(HPAIV),and no amino acid mutation converting to SAα2-6 Gal receptor gene was found.HA gene phylogenetic analysis results showed that the virus fell into 2.3.4 lineage,and NA gene belonged to Eurasian lineage.The genome of AIV in the inactivated cloacal swab was successfully sequenced by targeted nanopore sequencing technology,verifying that the technology was rapid and convenient,could be directly used for whole genome sequencing of viruses in swab samples and for rapid molecular identification and traceability of animal influenza viruses.关键词
纳米孔/靶向基因组测序/禽流感病毒/分子溯源/阳性样品Key words
nanopore/targeted genome sequencing/AIV/molecular traceability/positive sample分类
农业科技引用本文复制引用
高志强,汪琳,刘环,白子龙,赵相鹏,蒲静,张伟..纳米孔测序技术对一份禽流感病毒阳性样品的分子溯源[J].中国动物检疫,2024,41(2):86-91,6.基金项目
海关总署科研项目(2020HK178) （2020HK178）
国家重点研发计划项目(2021YFC2300904) （2021YFC2300904）
