中国动物检疫2024，Vol.41Issue(2)：92-98,7.DOI:10.3969/j.issn.1005-944X.2024.02.016
鸡传染性法氏囊病病毒VP5的原核表达、多抗制备及初步应用
Prokaryotic Expression of VP5 Protein of Infectious Bursal Disease Virus and Preparation and Preliminary Application of Its Polyclonal Antibodies
摘要
Abstract
Infectious bursal disease(IBD)is an acute infectious disease caused by infectious bursal disease virus(IBDV),mainly posing a threat to chicks.For 5 proteins encoded by IBDV genome,VP5 is the only one of which the gene may be deleted.In the study,the gene encoding VP5 protein(VP5 gene)of the dominant strain of IBDV was cloned,VP5 protein was expressed by prokaryotic expression system,and VP5 polyclonal antibodies were prepared through vaccinating BALB/c mice,then its application in various IBDV strains was identified and tested.The results showed that VP5 protein of IBDV dominant strain was expressed with solubility,and its molecular weight was about 17 kDa;the prepared VP5 polyclonal antibodies were with good specificity,and the ELISA titer could reach 1:64 000;VP5 polyclonal antibodies could identify IBDV rHLJ0504-HT strain through IFA and Western Blot,but fail for VP5 gene-deleted strain.In conclusion,VP5 protein of the dominant strain of IBDV was successfully expressed,and VP5 polyclonal antibodies were prepared,which could be used to identify and test IBDV wild and VP5 gene-deleted strain.A material basis was provided by the study for the development of IBDV detection methods and further research on the function of VP5 gene.关键词
传染性法氏囊病病毒/VP5/表达/多抗/应用Key words
IBDV/VP5/expression/polyclonal antibody/application分类
农业科技引用本文复制引用
韩金泽,韩京哲,吴子文,于晓雪,高玉龙,李留安,祁小乐,牛鑫鑫,王国栋,葛成菲,张玉龙,黄萌萌,许萌萌,于航博,刘润杭..鸡传染性法氏囊病病毒VP5的原核表达、多抗制备及初步应用[J].中国动物检疫,2024,41(2):92-98,7.基金项目
"十四五"黑龙江省重点研发计划项目(GA21B004) （GA21B004）
黑龙江省自然科学基金项目(ZD2020C006) （ZD2020C006）
国家自然科学基金项目(32072852) （32072852）
