中国动物检疫2024，Vol.41Issue(2)：99-102,4.DOI:10.3969/j.issn.1005-944X.2024.02.017
不同品牌胶体金免疫层析试纸条/卡检测牛羊布鲁氏菌病的效果分析
Analysis on the Effectiveness of Colloidal Gold Immunochromatographic Strips/Cards for Detecting Brucellosis in Cattle and Sheep
汪洪冰 1郭永祥 1张朝阳 1黄建龙 1张坤 1林源1
作者信息
- 1. 湖南省动物疫病预防控制中心,湖南长沙 410014
- 折叠
摘要
Abstract
In order to compare the effectiveness of colloidal gold immunochromatographic strips/cards with different brands for detecting brucellosis in cattle and sheep,6 brands of strips/cards were used to detect antibodies in Brucella negative and positive serum to compare their accuracy and sensitivity;and clinical serum samples that were preliminarily screened using Rose-bengal plate agglutination test(RBPT)and rechecked using compet0itive enzyme-linked immunosorbent assay(cELISA)were tested and compared by the strips/cards.The results showed that the detection rates of the strips/cards for enterprise-standard Brucella negative and positive serum samples were all 100%,in which two strips/cards had higher sensitivity and could detect positive serum with different dilution ratios;the strips/cards could detect 100%clinical negative serum samples,but the results for clinical positive serum samples were greatly different,with a detection rate of 5.88%～70.59%for sheep positive serum samples and 50.0%～100.0%for cattle positive serum samples.In conclusion,different brands of strips/cards were with different performance in detecting brucellosis.For the purpose of obtaining accurate test results,it was thus recommended that brucellosis should be reexamined using relevant national diagnostic techniques after it was preliminarily screened by the strips/cards.关键词
胶体金免疫层析法/布鲁氏菌病/效果分析Key words
colloidal gold immunochromatography/brucellosis/analysis on effectiveness分类
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汪洪冰,郭永祥,张朝阳,黄建龙,张坤,林源..不同品牌胶体金免疫层析试纸条/卡检测牛羊布鲁氏菌病的效果分析[J].中国动物检疫,2024,41(2):99-102,4.
