中国临床药理学杂志2024，Vol.40Issue(4)：539-543,5.DOI:10.13699/j.cnki.1001-6821.2024.04.013
莪术醇对肝星状细胞铁死亡和上皮间质转换作用的研究
Effects of curcumol on iron death and epithelial-mesenchymal transition in hepatic stellate cells
摘要
Abstract
Objective To elucidate the effect of curcumol on hepatic stellate cell iron death and epithelial-mesenchymal transition(EMT),and to investigate the molecular mechanism of its anti-liver fibrosis effect.Methods A model of hepatic stellate cell activation was constructed using normal cultured hepatic stellate cells in vitro,and the cells were divided into blank group and experimental-L,-M,-H groups.The blank group was given DMEM complete culture solution for normal culture;the experimental-L,-M,-H groups were given DMEM complete culture solution containing 12.5,25.0 and 50.0 mg·L-1 curcumol for 48 h of intervention.The effects of curcumol on the proliferation of hepatic stellate cells was observed by CCK-8.The expression levels of glutathione peroxidase 4(GPX4)and solute carrier family 7 member 11(SLC7A11)were detected by Western blot.The expression levels of E-cadherin and N-cadherin were detected by immunofluorescence.Results The cell proliferation rates of the experimental-M,-H groups and blank group were(68.97±5.61)％,(61.91±4.40)％and(118.07±10.01)％;the relative expression levels of GPX4 were 0.37±0.04,0.28±0.03 and 0.58±0.05;the relative expression levels of SLC7A11 were 0.38±0.04,0.28±0.03 and 0.60±0.05;E-cadherin levels were 6.76±1.09,9.57±1.73 and 2.05±0.72;N-cadherin levels were 5.66±0.66,3.44±0.78 and 10.37±0.66.The differences of above indicators were statistically significant between the blank group and the experimental-M,-H groups(P＜0.05,P＜0.01).Conclusion Curcumol promotes iron death in hepatic stellate cells,thereby inhibiting hepatic stellate cell EMT,which may be its molecular mechanism to prevent and treat liver fibrosis.关键词
莪术醇/肝星状细胞/铁死亡/上皮间质转换/肝纤维化Key words
curcumol/hepatic stellate cell/ferroptosis/epithelial-mesenchymal transition/liver fibrosis分类
医药卫生引用本文复制引用
汪磊,黄金彪,黄艳青,王泽宇,王佳慧,郑洋,罗伟生,赵铁建..莪术醇对肝星状细胞铁死亡和上皮间质转换作用的研究[J].中国临床药理学杂志,2024,40(4):539-543,5.基金项目
国家自然科学基金资助项目(81960751,82204755) （81960751,82204755）
广西壮瑶药重点实验室基金资助项目(GXZYYKF2023-05) （GXZYYKF2023-05）
广西中医药大学青年基金资助项目(2022MS024,2022QN008) （2022MS024,2022QN008）
广西中医药大学赛恩斯新医药学院科研基金资助项目(2022MS002,2022MS008,2022QJ001) （2022MS002,2022MS008,2022QJ001）
广西中医药大学赛恩斯新医药学院国家级大学生创新创业训练基金资助项目(202313643008) （202313643008）
