中国临床药理学杂志2024，Vol.40Issue(4)：574-578,5.DOI:10.13699/j.cnki.1001-6821.2024.04.020
右美托咪定对心肌缺血再灌注小鼠的保护作用
Protective effect of dexmedetomidine on myocardial ischemia-reperfusion mice
摘要
Abstract
Objective To investigate the protective effect of dexmedetomidine(Dex)pretreatment on myocardial ischemia-reperfusion mice and the effect of Nod-like receptor protein 3(NLRP3)inflammatory signaling pathway.Methods C57BL/6J mice were randomly divided into sham group(only threading without ligation),model group(recovery after ligation of left anterior descending coronary artery),positive group(modeling after intraperitoneal injection of 1 mg·kg-1 trimetazidine),Dex group(modeling after intraperitoneal injection of 20 μg·kg-1 dexmedetomidine),MCC950 group(modeling after intraperitoneal injection of 10 mg·kg-1 NLRP3 inhibitor MCC950),12 mice in each group.Cardiac function indexes were detected at 24 h after reperfusion,the expression level of related proteins in myocardial tissue was detected by Western blot,enzyme-linked immunosorbent assay(ELISA)was used to detect the expression level of serum factor,myocardial antioxidant index was detected by kit method,and apoptosis was detected by Tunel method.Results The NLRP3 protein expression levels of sham group,model group,positive group,Dex group and MCC950 group were 0.31±0.05,1.06±0.07,0.52±0.05,0.65±0.07 and 0.39±0.04,respectively;the expression levels of apoptosis-associated granuloid protein(ASC)were 0.27±0.08,0.88±0.09,0.46±0.05,0.59±0.07 and 0.34±0.04,respectively;CK-MB levels were(25.64±2.94),(102.08±7.04),(49.61±7.70),(60.86±5.24)and(63.24±5.38)U·L-1,respectively;IL-6 levels were(104.78±10.73),(231.54±15.56),(158.20±16.54),(165.10±14.77)and(141.17±14.08)pg·mL-1,respectively;Tunel positive cell rates were(4.34±0.16)％,(25.98±1.58)％,(8.74±0.93)％,(11.06±1.07)％and(9.19±0.88)％,respectively.Sham group were compared with model group;Dex group and MCC950 group were compared with model group,the above indexes were statistically significant(all P＜0.05).Conclusion Dexmedetomidine preconditioning may prevent ischemia-reperfusion myocardial injury by inhibiting NLRP3/ASC/caspase-1 inflammatory pathway,inflammatory response and myocardial cell apoptosis.关键词
右美托咪定/心肌缺血再灌注/炎性反应/心功能/Nod样受体蛋白3/凋亡相关颗粒样蛋白/胱天蛋白酶-1通路Key words
dexmedetomidine/myocardial ischemia-reperfusion/inflammatory response/heart function/Nod-like receptor protein 3/apoptosis-associated granuloid proteins/cysteine aspartate specific proteinase-1 pathway分类
医药卫生引用本文复制引用
胡振飞,黄一丹,戴晓雯..右美托咪定对心肌缺血再灌注小鼠的保护作用[J].中国临床药理学杂志,2024,40(4):574-578,5.基金项目
新疆维吾尔自治区自然科学基金资助项目(2019D01C324) （2019D01C324）
