中国临床药理学杂志2024，Vol.40Issue(4)：584-588,5.DOI:10.13699/j.cnki.1001-6821.2024.04.022
基于盲肠内容物代谢组学的炙红芪干预脾气虚大鼠的作用研究
Honey-processed Hedysari Radix in treatment of spleen-Qi deficiency rats based on metabonomics of the cecum contents
摘要
Abstract
Objective To find potential biomarkers and analyzing metabolic pathways of the treatment by honey-processed Hedysari Radix,the cecal contents of rats with spleen-Qi deficiency were used as samples for analysis.Methods Sixty male SD rats were randomly divided into blank,model,experimental and control groups.The rats in other groups except the control group were carried out by using the three-factor compound modeling method of bitter-cold diarrhea,excessive exertion and hunger and satiety disorders.Experimental group was given 12.60 g·kg-1 honey-processed Hedysari Radix;control group was given 0.63 g·kg-1 lactobacillus bifidum triplex tabletsa;control and model groups received with equal volume of distilled water for a total of 15 days.Measure body weight,anal temperature,immune organ index of rats.Ultra-pressure liquid chromatography-quadrupole-exactive-mass spectrometry technology was used to measure the levels of endogenous metabolites in cecum contents.Orthogonal partial least squares discriminant analysis and database"Kyoto Encyclopedia of Genes and Genomes"were used to identify potential differential metabolites and possible metabolic pathways.Results After the intervention,the average body weight of the experimental,control,model and blank groups was(216.87±7.85),(210.96±9.03),(159.47±5.18)and(293.51±22.98)g;anal temperature was(36.14±0.48),(35.40±0.64),(34.50±0.78)and(36.61±0.34)℃;the thymus indexes were(1.19±0.20),(1.24±0.25),(0.47±0.15)and(1.31±0.21)mg·g-1;the spleen indexes were(1.95±0.33),(2.18±0.28),(1.61±0.27)and(2.29±0.24)mg·g-1.Compared with the model group,the above indexes of the experimental group and the control group were significantly increased(all P＜0.01).A total of 14 potential biomarkers of Honey-processed Hedysari Radix in treating spleen-Qi deficiency syndrome were screened out in this study,which mainly involved amino acid metabolism such as tryptophan and glutamate,riboflavin metabolism and adenosine 5'-monophosphate-activated protein kinase metabolism.Conclusion Honey-processed Hedysari Radix can further protect the intestinal mucosal barrier and reduce the intestinal inflammatory response by improving the metabolic level of cecum contents in rats with spleen-Qi deficiency in cecum contents,thus exerting the effect of strengthening the spleen and tonifying the Qi.关键词
炙红芪/脾气虚证/盲肠内容物/代谢组学Key words
honey-processed Hedysari Radix/spleen-Qi deficiency syndrome/cecum content/metabonomics分类
医药卫生引用本文复制引用
孙宇靖,宋沁洁,王彦钧,边甜甜,张育贵,李咸慰,李国峰,李越峰..基于盲肠内容物代谢组学的炙红芪干预脾气虚大鼠的作用研究[J].中国临床药理学杂志,2024,40(4):584-588,5.基金项目
国家自然科学基金资助项目(81960713,82160750,81460611) （81960713,82160750,81460611）
甘肃省教育厅产业支撑计划基金资助项目(2021CYZC-21) （2021CYZC-21）
甘肃省中药制药工艺工程研究中心开放课题基金资助项目(ZYGY202003) （ZYGY202003）
甘肃省科技计划(创新基地与人才计划)基础研究创新群体基金资助项目(21JR7RA569) （创新基地与人才计划）
