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不同玉米杂交种及其亲本自交系耐热性的差异比较OA北大核心CSTPCD

Comparison of Heat Tolerance of Maize Hybrids and Their Parental Inbreds with Different Genotypes

中文摘要英文摘要

[目的]近年来,我国黄淮海夏玉米区高温热害不利天气频发重发,已成为威胁玉米生产的重要不利因素.研究并明确高温胁迫对不同基因型玉米品种雌雄穗及产量的影响,为耐高温玉米品种培育和选择提供参考.[方法]以郑单 958(郑58×昌 7-2)、先玉 335(PH6WC×PH4CV)、京农科 728(京MC01×京 2416)和MC812(京B547×京 2416)及其亲本自交系为供试材料,花期前后(大喇叭口期-吐丝后 7 d)进行高温胁迫处理,研究高温胁迫对不同基因型玉米杂交种及其亲本自交系雌雄穗生长发育、散粉吐丝间隔(ASI)、花粉活性、产量及其构成要素的影响.[结果](1)花期前后高温胁迫导致参试玉米品种及其亲本自交系的穗粒数减少,进而产量降低,且不同品种和自交系对高温胁迫的响应存在显著差异.与对照相比,高温胁迫下,郑单958、先玉335、京农科728和MC812的穗粒数降幅分别为22.28%、46.79%、6.13%和8.11%,产量降幅分别为9.50%、50.61%、3.18%和5.00%,其中,京农科728和MC812减产不显著,而郑单958和先玉335显著减产;亲本自交系中,京 2416 的穗行数、行粒数和产量降幅最小且不显著,而PH6WC均显著降低且降幅最大.(2)高温胁迫下,参试品种及其亲本自交系的雄穗分支总数、雄穗长度、总散粉量和花粉活性降低,散粉持续期缩短,ASI延长.与对照相比,高温胁迫下,ASI延长 1.6 d.雄穗长度降幅表现为郑单 958>先玉 335>MC812>京农科 728.郑单 958 雄穗长度降幅最大,但雄穗分支数多,花粉量最大;先玉 335 雄穗分支少,雄穗长度降幅大,花粉量最少且活性最低;京 2416 总散粉量大且花粉活力强,降幅最小(仅为 4.50%和 3.98%).[结论]花期前后高温胁迫对参试玉米品种的籽粒产量和花粉活性等影响较大,京农科728和MC812的产量和花粉活性降幅显著低于先玉335和郑单958,耐热性较好.通过比较参试玉米杂交种亲本自交系的耐热性,父本自交系的耐热性要好于母本自交系.其中,京 2416 的雄穗分支和长度降幅小,花粉量大,花粉活性高,且花丝茸毛多,捕获花粉能力强,单穗产量高,ASI小,耐热性最好.因此,在耐高温玉米品种选育过程中,要重视和加大对耐热种质(如京2416等)的利用与改良创新,组配选育耐高温玉米新品种.

[Objective]In recent years,the adverse weather of high temperature and heat damage in the Huang-Huai-Hai maize region of China occurred frequently,which has become an important adverse factor threatening maize production.Study and clarify the effects of high temperature stress on male and female ear characteristics and yield of maize can provide useful guidance for the cultivation and selection of high temperature tolerant maize varieties.[Method]The variety of Zhengdan958(Zheng58×Chang7-2),Xianyu335(PH6WC×PH4CV),Jingnongke728(JingMC01×Jing2416),MC812(JingB547×Jing2416),and their parents were used as the test materials.High temperature stress before and after flowering(from V12 stage to 7 d after silking)were conducted.The effects of high temperature stress on the growth and development of male and female panicles,ASI,pollen activity,yield and yield components of different genotypes of maize hybrids and their parents were studied.[Result]High temperature stress before and after anthesis significantly reduced the ear length,rows per ear and grains per row of the tested maize varieties and their parents,and then resulted significant decrease in yield.Compared with the control,the grain number per spike of Zhengdan958,Xianyu335,Jingnongke728 and MC812 decreased by 22.28%,47.69%,6.13%and 8.11%respectively under high temperature stress,resulting yield decrease of 9.50%,50.61%,3.17%and 5.00%respectively.Among the parental materials,the decrease of rows per panicle,grains per row and yield of Jing2416 under high temperature treatment was the smallest and not significant,while the decrease of PH6WC was the largest.Under high temperature stress,the total number of tassel branches,the length of tassel,the total amount of loose pollen and pollen activity decreased significantly,the silking period of loose pollen was prolonged,and the duration of loose pollen was shortened.Among them,Jingnongke728 had the smallest decline,followed by MC812,showing good heat resistance,while Zhengdan958 had the largest decline in the length of tassel,but the amount of pollen was the largest due to the large number of tassel branches.Xianyu335 has fewer male panicle branches,a large decrease in male panicle length,the least amount of pollen and low activity.Among the parental materials,Jing2416 had a large amount of total loose pollen and strong pollen vitality under high temperature treatment,with the smallest decline,only 4.50 and 3.98 percentage points.Compared with the control,the interval of loose pollen silking(ASI)was prolonged by 1.6d under high temperature stress.The decrease in male spike length is manifested as Zhengdan958>Xianyu335>MC812>Jingnongke728.Zhengdan958 had the largest decrease in male spike length,but had more branches and the largest pollen yield;Xianyu335 has fewer branches of male spikes,a significant decrease in male spike length,the least pollen quantity,and the lowest activity;Jing 2416 has a large amount of loose pollen and strong pollen vitality,with the smallest decrease(only 4.50%and 3.98%).[Conclusion]High temperature stress before and after anthesis has a significant impact on the grain yield,male and female ear development process,pollen activity and filament microstructure of the tested maize varieties.Under high temperature stress at this stage,the decline of yield and pollen activity of Jingnongke728 and MC812 is significantly less than Xianyu335,showing higher single ear yield and heat tolerance.By comparing the heat resistance of the parental inbred lines of the tested maize hybrids,it was found that the heat resistance of the paternal inbred lines was better than that of the maternal inbred lines.The male panicle branch and length of the parent material Jing2416 decreased slightly,the amount of pollen was large,the pollen activity was high,the filaments were hairy,the ability to capture pollen was strong,the single panicle yield was high,and the heat resistance was the best.Therefore,in the planting area with frequent high temperature and heat damage,selecting maize varieties such as Jingnongke728 can achieve stable and high yield;and during the maize breeding process,we should pay more attention to the utilizing of the higher temperature resistant inbred such an Jing2416 and then combination higher temperature maize varieties.

徐田军;邢锦丰;赵久然;王荣焕;吕天放;李自豪;张勇;刘宏伟;刘月娥;蔡万涛;张如养;宋伟

北京市农林科学院玉米研究所/玉米DNA指纹及分子育种北京市重点实验室,北京 100097

高温玉米亲本自交系耐热性

high temperaturemaizeparental inbred linesheat resistance

《中国农业科学》 2024 (002)

403-415 / 13

国家重点研发计划课题任务(2021YFD1200701-8)、北京市农林科学院青年科研基金(QNJJ202236)

10.3864/j.issn.0578-1752.2024.02.014

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