中国农业科学2024，Vol.57Issue(2)：416-428,13.DOI:10.3864/j.issn.0578-1752.2024.02.015
红皮白肉与红皮红肉火龙果植株茎部内生微生物群落结构特征
Characteristics of Endophytic Microbial Community Structures in Stems Between Hylocereus undatus and H.polyrhizus
摘要
Abstract
[Objective]The differences of the endophytic microbial community structures between Hylocereus undatus and H.polyrhizus were analyzed,so as so to explore the correlation between the pulp color of H.polyrhizus and the endophytic microbial community composition and their functional microorganisms.[Method]Based on high-throughput sequencing technology,the diversity and richness of endophytic microorganisms(bacteria and fungi)in stems between H.undatus and H.polyrhizus were analyzed.Meanwhile,based on LEfSe analysis,the differences of endophytic microorganisms in stems between H.undatus and H.polyrhizus were also investigated.[Result]The significant differences of the endophytic microbial community structures were found in stems between H.undatus and H.polyrhizus.Meanwhile,the numbers of specific bacterial and fungal OTUs in stems of H.polyrhizus were all higher than those of H.undatus.At the phylum level,the relative abundance ratio of Ascomycota in stems of H.polyrhizus was 1.15 times higher than that of H.undatus.At the genus level,Streptomyces and Penicillium were the highest abundant dominant bacterial and fungal genera in stems of H.polyrhizus,which were 1.24 and 4.27 times higher than those of H.undatus,respectively.In addition,some bacterial genera,such as Lechevalieria,Glycomyces,unclassified_f__Enterobacteriaceae,Actinomadura,and some fungal gerera,such as Talaromyces,unclassified_f__Serendipitaceae,unclassified_c__GS13,unclassified_o__Atractiellales,unclassified_o__Auriculariales were enriched in stems of H.polyrhizus.LEfSe analysis also showed that Promicromonospora and Xylomyces were significant enriched in stems of H.polyrhizus.[Conclusion]All above results suggested that the formation of pigment was closely related to the compositions of endophytic microbial community in stems of H.polyrhizus.The bacterial genera,such as Streptomyces,unclassified_f__Enterobacteriaceae,Promicromonospora,and the fungal phylum and genera,such as Ascomycota,Penicillium,Talaromyces and Xylomyces,were all the potential microorganisms in relating to pigment synthesis and metabolic accumulation in stems of H.polyrhizus.关键词
火龙果/果肉颜色/内生微生物/高通量测序Key words
Pitaya(Hylocereus undulatus Britt)/pulp color/endophytic microorganisms/high-throughput sequencing引用本文复制引用
周信雁,陈思宇,魏宇飞,朱瑜,冯君茜,丁点草,陆贵锋,杨尚东..红皮白肉与红皮红肉火龙果植株茎部内生微生物群落结构特征[J].中国农业科学,2024,57(2):416-428,13.基金项目
广西重点研发计划(桂科AB 19245012)、广西研究生教育创新计划资助项目(YCSW2023038) （桂科AB 19245012）
