中国临床药理学杂志2024，Vol.40Issue(3)：393-397,5.DOI:10.13699/j.cnki.1001-6821.2024.03.019
果王素抗大鼠肺纤维化的作用
Effects and mechanism of Kiwi fruit essence on pulmonary fibrosis in rats
摘要
Abstract
Objective To observe the effects of different doses of Kiwi fruit essence on fibroblast growth factor 2(FGF2)and extracellular regulatory protein kinase 1/2(ERK1/2)in lung tissue of rats with pulmonary fibrosis.Methods SD rats were divided into blank control group(0.9％NaCl),model group(0.9％NaCl),positive control group(5 mg·kg-1 prednesone acetate),experimental group-L,-M,-H(60,120,180 mg·kg-1 Kiwi fruit essence),except the blank control group was injected with 0.9％NaCl at one time.The other groups were injected 5 mg·kg-1 bleomycin A5 into the trachea to construct the rat pulmonary fibrosis model.The expressions of FGF2 and ERK1/2 proteins and mRNA were detected by Western blot and real-time fluorescence quantitative polymerase chain reaction.Results The relative expression levels of FGF2 protein in blank control group,model group,positive control group and experimental group-L,-M,-H groups on the day 28 were 0.07±0.01,0.63±0.15,0.14±0.02,0.16±0.01,0.26±0.02 and 0.44±0.03,respectively;the relative expression levels of ERK1/2 protein on day 28 were 0.06±0.00,0.41±0.05,0.09±0.00,0.10±0.00,0.14±0.02,0.21±0.02,respectively;the relative expression levels of FGF2 mRNA on day 28 were 0.98±0.05,9.32±1.03,2.21±0.32,2.87±0.44,5.32±0.21,6.37±0.66,respectively;the relative expression levels of ERK1/2 mRNA on day 28 were 1.00±0.01,9.22±0.85,1.93±0.63,2.36±0.54,5.34±0.44 and 6.85±1.09,respectively.The above indexes in the model group were significantly different from those in the blank control group(all P＜0.05).The above indexes in the positive control group and the experimental-L,-M,-H groups were significantly different from those in the model group(all P＜0.05).Conclusion Kiwi essence may inhibit pulmonary fibrosis by regulating the expression of FGF2 and ERK1/2.关键词
果王素/肺纤维化/成纤维细胞生长因子2/细胞外调节蛋白激酶1/2Key words
Kiwi fruit essence/pulmonary fibrosis/fibroblast growth factor-2/extracellular-regulatory protein kinase 1/2分类
医药卫生引用本文复制引用
陈荟婷,魏宁宁,董晓军,黄民江..果王素抗大鼠肺纤维化的作用[J].中国临床药理学杂志,2024,40(3):393-397,5.基金项目
湖南省自然科学基金资助项目(2021JJ40385) （2021JJ40385）
湖南省教育厅一般基金资助项目(22C1182) （22C1182）
