中国临床药理学杂志2024，Vol.40Issue(3)：398-402,5.DOI:10.13699/j.cnki.1001-6821.2024.03.020
益母草碱对结肠癌荷瘤小鼠肿瘤生长和免疫功能的影响
Impacts of leonurine on tumor growth and immune function in colon cancer bearing mice
摘要
Abstract
Objective To investigate the effects of leonurine on tumor growth and immune function of colon cancer tumor bearing mice.Methods C57BL/6 mice were established a colon cancer bearing mouse model.After modeling,the mice were randomly divided into control group(0.9％NaCl),experimental-L group(180 ng·g-1 leonurine),experimental-H group(360 ng·g-1 Leonurine),experimental-H+NC group(360 ng·g-1 leonurine+no-load plasmid),and experimental-H+sicGAS group(360 ng·g-1 leonurine+cGAS siRNA plasmid).Tumor volume and weight were measured after group treatment.Immunohistochemical staining was used to detect the relative positive expression of cyclic GMP-AMP synthase(cGAS),stimulator of interferon gene(STING)and the average positive number of CD4+T and CD8+T cells in tumor tissues.The killing rate of mouse CD4+T and CD8+T cells to tumor cells was detected by cell counting kit 8.Results The tumor volume of control group,experimental-H group,experimental-H+NC group and experimental-H+cGAS knockout group were(1 088.32±70.82),(468.20±24.28),(480.01±23.78)and(998.99±32.84)mm3,respectively;the relative positive expressions of cGAS were 1.00±0.00,5.18±0.73,5.30±0.80 and 1.12±0.46,respectively;the relative positive expressions of STING were 1.00±0.00,5.64±0.81,5.48±0.75 and 1.14±0.50,respectively;the mean CD4+T positive cells were 40.25±5.82,118.64±15.30,113.25±16.65 and 48.74±7.10,respectively;the average number of CD8+T positive cells were 49.76±6.12,143.68±16.80,135.75±17.42 and 57.01±8.24,respectively.The above indexes in experimental-H group and experimental-H+no-load group were statistically significant compared with control group(all P＜0.05);there were statistically significant differences in the above indexes between the experimental-H+sicGAS group and the experimental-H group(all P＜0.05).Conclusion Leonurine can enhance the proliferation ability of lymphocytes in colon cancer tumor bearing mice by activating cGAS-STING signal,and inhibit tumor growth in mice.关键词
益母草碱/环状GMP-AMP合成酶/干扰素基因刺激因子/结肠癌/荷瘤/肿瘤生长/免疫功能Key words
leonurine/cyclic GMP-AMP synthetase/stimulator of interferon gene/colon cancer/tumor bearing/tumor growth/immune function分类
医药卫生引用本文复制引用
刘建邦,黄娟,李斌..益母草碱对结肠癌荷瘤小鼠肿瘤生长和免疫功能的影响[J].中国临床药理学杂志,2024,40(3):398-402,5.基金项目
国家卫生健康委"十四五"规划全国重点课题基金资助项目(YYWS2810) （YYWS2810）
