中国畜牧杂志2024，Vol.60Issue(2)：157-162,6.DOI:10.19556/j.0258-7033.20220816-04
鸡SLMO2基因CDS区克隆、生物信息学及组织表达分析
Cloning,Expression and Bioinformatics Analysis of the CDS Region of Chicken SLMO2 Gene
摘要
Abstract
This study is to investigate the amino acid sequence characteristics of drosophila-like slow-moving gene and its mRNA expression in different tissues of chicken with different skin colors.The CDS sequence of SLMO2 with a total length of 560 bp was cloned by PCR,and it encoded 174 amino acids.The bioinformatics analyses showed that there were no transmembrane structures and signal peptides in the chicken SLMO2 protein,and the protein structure was mainly composed of irregular curly and α-spiral structures,and it was distributed in the mitochondrial inner membrane space.Amino acid sequence comparison showed that the genetic relationship between chicken and Xenopus was close.qRT-PCR assay showed that the expression level of SLMO2 in the back skin tissue of Silkie was significantly higher than that of other tissues(P<0.01),and the expression level of SLMO2 in the back skin tissue of yellow-feather chicken was significantly higher than that in other tissues(P<0.01).The above results will provide a theoretical basis for further study on the regulatory mechanism of chicken SLMO2 gene in melanin deposition.关键词
鸡/SLMO2基因/CDS区克隆/组织表达/生物信息学分析Key words
Chicken/SLMO2 gene/CDS region cloning/Expression analysis/Bioinformatics analysis分类
农业科技引用本文复制引用
朱慧媛,康相涛,李东华,张朋威,徐硕辉,李瑞婷,高超群,李文婷,蒋瑞瑞,孙桂荣,韩瑞丽..鸡SLMO2基因CDS区克隆、生物信息学及组织表达分析[J].中国畜牧杂志,2024,60(2):157-162,6.基金项目
河南省自然科学基金青年项目(202300410203) （202300410203）
国家自然科学基金项目(32102540) （32102540）
中原学者首席科学家工作室(30601985) （30601985）
