中国临床药理学杂志2024，Vol.40Issue(3)：413-418,6.DOI:10.13699/j.cnki.1001-6821.2024.03.023
基于UPLC-Q-TOF/MS探讨感咳方药效物质基础及作用机制的研究
Study on therapeutic material basis and mechanism of Ganke Formula based on UPLC-Q-TOF/MS
摘要
Abstract
Objective To explore the therapeutic material basis and antitussive mechanism of Ganke Formula.Methods Ultra-high performance liquid chromatography-quadrupole-time of flight mass spectrometry(UPLC-Q-TOF/MS)technology was used to analyze and identify the components of Ganke Formula in serum.30 rats were randomly divided into control group,model group,experimental-L group,experimental-M group and experimental-H group,6 rats per group.Acute bronchitis rat model was established by smoke inhalation and cold stimulus.The experimental-L,-M,-H groups were respectively given 1.86,4.66,9.32 g·kg-1(crude drug/weight)dose of Ganke Formula per day by intragastric administration for 7 days.The control group and model group were given the equal amount of normal saline.The contents of interleukin-1β(IL-1β)and tumor necrosis factor-α(TNF-α)in rat lung tissue were determined by enzyme-linked immunosorbent assay.The expressions of phosphatidylinositol 3-kinase(PI3K)and phosphorylated protein kinase B(p-Akt)in lung were detected by Western blotting.Results Twenty-six absorbed components have been identified by UPLC-Q-TOF/MS.The contents of IL-1β in control group,model group,experimental-M,-H group were(57.80±7.67),(186.48±8.50),(166.05±9.90)and(143.19±6.31)pg·mL-1;TNF-α contents were(47.14±8.55),(316.22±9.49),(68.93±7.94)and(65.93±7.10)pg·mL-1;the relative expression levels of PI3K in lung tissues were 0.38±0.05,0.97±0.10,0.68±0.15 and 0.56±0.10;the relative expression levels of p-Akt were 0.34±0.14,0.93±0.05,0.63±0.16 and 0.49±0.14,respectively.Compared with the model group,the above indicators in the experimental-H group and control group were statistically different(P＜0.01 or P＜0.05).Conclusion Ganke Formula can intervene in the expression of inflammatory and immune regulatory factors by regulating PI3K/Akt signaling pathway,improve airway inflammation,and thus exert cough relieving effects.关键词
感咳方/急性支气管炎/超高效液相色谱-四极杆-飞行时间质谱/药效物质基础/网络药理学/作用机制Key words
Ganke Formula/acute bronchitis/ultra-high performance liquid chromatography-quadrupole-time of flight mass spectrometry/therapeutic material basis/network pharmacology/mechanism分类
医药卫生引用本文复制引用
张雪,袁强华,何成诗,徐娜,陈小睿,张敏..基于UPLC-Q-TOF/MS探讨感咳方药效物质基础及作用机制的研究[J].中国临床药理学杂志,2024,40(3):413-418,6.基金项目
四川省中医药管理局中医药重大科技基金资助项目(2021XYCZ005) （2021XYCZ005）
