中国实用内科杂志2024，Vol.44Issue(1)：68-74,7.DOI:10.19538/j.nk2024010113
骨髓增生异常综合征CD34+细胞焦亡相关分子焦孔素E研究
GSDME,a molecule related to pyroptosis of CD34+cells in patients with myelodysplastic syndrome
摘要
Abstract
Objective To investigate the expression levels of GSDME and Caspase-3 in bone marrow CD34+cells of myelodysplastic syndrome(MDS)patients.Methods From September 2020 to December 2021,20 normal controls and 32 patients with myelodysplastic syndrome admitted to the hematology department of Tianjin Medical University General Hospital were compared,expression of GSDME and Caspase-3 in bone marrow CD34+cells was detected by Flow Cytometry and qRT-PCR.Results According to the Flow Cytometry,the expression levels of GSDME and Cleaved Caspase-3 of CD34+cells in bone marrow of low risk group were increased[(64.90±15.74)％,(31.83±17.99)％]than the control group[(41.39±14.07)％,(24.11±16.28)％,P＜0.05];The expression of GSDME and Cleaved Caspase-3 in the high risk group[(30.69±14.62)％,(15.42±13.34)％,P＜0.05]were decreased.According to the result of qRT-PCR,the relative expression of GSDME(0.20±0.19 vs.1.00±0.53,P＜0.05)gene in high risk MDS patients was significantly decreased,but there was no significant difference in the expression of Caspase-3.Conclusion In low risk MDS patients,the expression of GSDME in CD34+cells increase,the level of pyroptosis may increase,leading to bone marrow hematopoietic failure.In high risk MDS patients,the expression of GSDME in CD34+cells decrease,the level of pyroptosis may decrease,the cells may proliferate excessively,and the risk of leukemia transformation is increased.关键词
骨髓增生异常综合征/细胞焦亡/焦孔素E/胱天蛋白酶-3Key words
myelodysplastic syndrome/pyroptosis/GSDME/Caspase-3分类
医药卫生引用本文复制引用
任晓彤,余顺杰,郭欣雨,刘召云,邵宗鸿,付蓉,李丽娟..骨髓增生异常综合征CD34+细胞焦亡相关分子焦孔素E研究[J].中国实用内科杂志,2024,44(1):68-74,7.基金项目
天津市医学重点学科(专科)建设项目(TJYXZDXK-028A) （专科）
