中国康复理论与实践2024，Vol.30Issue(2)：176-182,7.DOI:10.3969/j.issn.1006-9771.2024.02.006
丰富环境训练结合头穴丛刺对孤独症谱系障碍模型大鼠行为的影响
Effect of enriched environment combined with acupuncture at head points on behavior in rats with autism spec-trum disorder
摘要
Abstract
Objective To observe the effect of enriched environment(EE)combined with acupuncture at head point(HA)on behav-ior in rats with autism spectrum disorder. Methods Healthy female Wistar rats were given peritoneal injection of sodium valproate at 12.5 days of gestation.Twen-ty-four male offspring rats were randomly selected and then randomly divided into model group(n = 6),EE group(n = 6),HA group(n = 6)and EE combined with HA group(the combined group,n = 6).Six male off-spring rats born from female mice injected with the same amount of saline intraperitoneally were as control group.After four weeks of treatment,all the five groups were tested with three-chamber test and marble burying test,and the sociability index,the social novelty index and the number of buried marbles were recorded.The lev-els of interleukin(IL)-1β and IL-6 in peripheral blood were determined by enzyme-linked immunosorbent assay(ELISA). Results After treatment,compared with the model group,the sociability index and the social novelty index improved(P<0.05),the number of buried marbles reduced(P<0.05),and the levels of IL-6 and IL-1β in peripheral blood decreased in EE group,HA group and the combined group(P<0.05);while the combined group was the best(P<0.01). Conclusion Both EE or acupuncture at HA could improve behavioral symptoms,and reduce the expression of inflamma-tory factors in rats with autism spectrum disorder.The combination of the two methods showed the best result.关键词
孤独症谱系障碍/头穴丛刺/丰富环境/行为/大鼠Key words
autism spectrum disorder/acupuncture at head point/enriched environment/behavior/rats分类
医药卫生引用本文复制引用
穆姿辰,张春艳,袁孟珂,唐强,史云秋,王艳,朱淑伟,庄亚楠,徐丹双,李宏玉,李保龙..丰富环境训练结合头穴丛刺对孤独症谱系障碍模型大鼠行为的影响[J].中国康复理论与实践,2024,30(2):176-182,7.基金项目
黑龙江省自然科学基金项目(No.LH2020H079) Natural Science Foundation of Heilongjiang(No.LH2020H079) （No.LH2020H079）
