中国药科大学学报2024，Vol.55Issue(1)：137-146,10.DOI:10.11665/j.issn.1000-5048.2024011701
新型冠状病毒疫苗Ad5-nCoV不同给药途径异源加强接种的系统免疫原性比对分析
A comparative analysis of vaccine immunity induced by heterologous booster with Ad5-nCoV via different routes of administration
摘要
Abstract
Heterologous boost COVID-19 vaccination can solved the problem of decreased efficacy caused by single dose of vaccine.Heterologous booster with adenoviral-vectored COVID-19 vaccine(Ad5-nCoV)following primary immunization with inactivated COVID-19 vaccines is a widely-used vaccination strategy in clinic,while different routes of Ad5-nCoV administration exist and pose a question which route could be more optimal.In this study,we comprehensively evaluated and compared the vaccine immunity induced in mice immunized according to three different vaccination regimens:"3×phosphate buffered solution(3×PBS)","2×inactivated vaccine + 1×inactivated vaccine(3×INA)","2×inactivated vaccine + 1×Ad5-nCoV(intramuscular)[2×INA+Ad5(im)]"and"2×inactivated vaccine + 1×Ad5-nCoV(intranasal)[2×INA+Ad5(in)]".We found that heterologous booster with Ad5-nCoV,irrespective of the route of administration,induced significantly higher levels of anti-Spike IgG and subclasses(IgG1and IgG2c),Spike-specific T cells,class-switched Spike+ memory B cells(MBCs)than homologous booster with 3rd dose of inactivated COVID-19 vaccine.Of note,compared with the intramuscular given,intranasal given of Ad5-nCoV as a booster dose clearly induced higher levels of serum and bronchoalveolar bavage fluid anti-spike immunoglobulin A,and moreover,induced stronger infiltration of major innate effector cells like neutrophils,natural killer cells and dendritic cells into the lung tissue,which suggested that mucosal vaccine responses are generated upon intranasal booster with Ad5-nCoV.Altogether,our study analyzed the vaccine immunity induced by different COVID-19 vaccines administered using different regimens,which may guide the clinical use of other types of prophylactic vaccines aiming to mount improved vaccine responses.关键词
新型冠状病毒疫苗/异源加强接种/同源加强接种/给药途径/免疫原性/黏膜免疫Key words
COVID-19 vaccines/heterologous booster/homologous booster/route of administration/immunogenicity/mucosal response分类
医药卫生引用本文复制引用
马文煊,韩雨红,林昂,赵维俊..新型冠状病毒疫苗Ad5-nCoV不同给药途径异源加强接种的系统免疫原性比对分析[J].中国药科大学学报,2024,55(1):137-146,10.基金项目
国家自然科学基金项目(No.32200764) （No.32200764）
江苏省自然科学基金项目(No.BK20221031)This study was supported by the National Natural Science Foundation of China(No.32200764)and the Natural Science Foundation of Jiangsu Province(No.BK20221031) （No.BK20221031）
