中国临床药理学杂志2024，Vol.40Issue(4)：529-533,5.DOI:10.13699/j.cnki.1001-6821.2024.04.011
槲皮素介导铁死亡途径对高渗刺激诱导的干眼细胞模型的保护作用
Protective effect of quercetin mediated ferroptosis pathway on hypertonic stimulation induced dry eye cell model
摘要
Abstract
Objective To investigate the effect of quercetin on HCE-2 injury of human corneal epithelial cells induced by high osmotic pressure and its mechanism.Methods HCE-2 cells were randomly divided into control group(normal osmotic pressure),model group(high osmotic pressure),experimental-L group(high osmotic pressure+31.25 pg·mL-1 quercetin),experimental-M group(high osmotic pressure+62.50 μg·mL-1 quercetin),experimental-H group(high osmotic pressure+125.00 μg·mL-1 quercetin),erastin group(high osmotic pressure+125.00 μg·mL-1 quercetin+30.00 μmol·L-1 iron death inducer erastin).Cell survival rate was detected by cell counting kit 8;reactive oxygen species(ROS)levels was detected by C11-BODIPY 581/591 probe staining;glutathione(GSH)and malondialdehyde(MDA)levels were determined by kit method;the expression levels of glutathione peroxidase 4(GPX4),dihydrolactate dehydrogenase(DHODH)and ferroptosis suppressor protein 1(FSP1)were detected by real-time quantitative polymerase chain reaction and Western blot.Results The cell survival rates of control group,model group,experimental-H group and erastin group were(100.00±3.97)％,(50.05±5.83)％,(86.35±7.35)％and(58.32±4.66)％,respectively;ROS levels were 1.00±0.09,2.45±0.16,1.19±0.05 and 2.09±0.30,respectively;GPX4 protein levels were 1.09±0.11,0.34±0.03,0.91±0.12 and 0.30±0.04,respectively;FSP1 protein levels were 0.92±0.06,0.25±0.03,0.89±0.07 and 0.39±0.07,respectively;DHODH protein levels were 0.89±0.11,0.31±0.04,0.86±0.11,0.41±0.04,respectively.Compared with model group,the above indexes in control group were statistically significant(all P＜0.05);the differences between experimental-H group and model group were statistically significant(all P＜0.05);the above indexes in erastin group were significantly different from those in experimental-H group(all P＜0.05).Conclusion Quercetin can ameliorate HCE-2 cell damage induced by high osmotic pressure by inhibiting iron death pathway.关键词
槲皮素/干眼病/铁死亡/人角膜上皮细胞/脂质过氧化Key words
quercetin/dry eye disease/ferroptosis/human corneal epithelial cells/lipid peroxidation分类
医药卫生引用本文复制引用
王佳娣,安百平,刘悦,曹丛红,宗贝婷,姚靖..槲皮素介导铁死亡途径对高渗刺激诱导的干眼细胞模型的保护作用[J].中国临床药理学杂志,2024,40(4):529-533,5.基金项目
国家自然科学基金面上基金资助项目(81973908) （81973908）
黑龙江省中医药科研基金资助项目(ZYW2022-051) （ZYW2022-051）
黑龙江省中医药科研基金资助项目(ZHY2023-126) （ZHY2023-126）
