中国中医药信息杂志2024，Vol.31Issue(3)：132-137,6.DOI:10.19879/j.cnki.1005-5304.202307337
HPLC指纹图谱结合多指标含量测定的抵当芪桂汤质量评价研究
Study on Quality Evaluation of Didang Qigui Decoction by HPLC Fingerprint Combined with Multi-component Content Determination
摘要
Abstract
Objective To establish an HPLC fingerprint of Dingdang Qigui Decoction and analyze and evaluate it using chemical pattern recognition technology;To determine the contents of 5 effective chemical components in Dingdang Qigui Decoction;To provide a basis for its quality control.Methods The analysis was performed on Agilent 5 TC-C18(2)column(250 mm×4.6 mm).The mobile phase comprised of acetonitrile-0.1%phosphoric acid aqueous solution with the gradient elution at a flow rate of 1.0 mL/min.The detection wavelength was set at 260 nm.The column temperature was maintained at 30℃and the injection volume was 10 μL.SPSS 26.0 and SIMCA 14.1 were used to perform clustering analysis and principal component analysis on the 10 batches of Didang Qigui Decoction.The landmark components for inter batch differences were selected through orthogonal partial least squares discriminant analysis(OPLS-DA).Results The HPLC fingerprint with eighteen common peaks of Didang Qigui Decoction in 10 batches of sample was established,and the similarities of samples were between 0.828 and 0.989.Five indicative components were identified and quantitatively analyzed by comparing with the reference substances,which were paeoniflorin,mauroisoflavone glucoside,hesperidin,cinnamaldehyde and aloe rhodopsin.The linear ranges was 10.000 0-320.000 0 μg/mL,2.500 0-80.000 0 μg/mL,10.000 0-320.000 0 μg/mL,10.000 0-320.000 0 μg/mL,0.078 1-5.000 0 μg/mL,respectively,and their mean recovery ranged from 100.30%to 104.09%.Clustering analysis and principal component analysis divided 10 batches of samples from Didang Qigui Decoction into 2 categories.Through OPLS-DA screening,hairy pistil isoflavone glycosides,paeoniflorin,and hesperidin were selected as landmark components for quality differences.Conclusion The quality evaluation method for Didang Qigui Decoction established in this study is simple,sensitive,accurate,and reproducible,which can provide a basis for the quality evaluation of Didang Qigui Decoction.关键词
抵当芪桂汤/高效液相色谱法/指纹图谱/质量评价Key words
Didang Qigui Decoction/HPLC/fingerprint/quality evaluation分类
医药卫生引用本文复制引用
郭怡佳,程都,张潇,雷莉妍,梁艳妮,王征,杨景锋..HPLC指纹图谱结合多指标含量测定的抵当芪桂汤质量评价研究[J].中国中医药信息杂志,2024,31(3):132-137,6.基金项目
陕西省重点研发计划(2018ZDCXL-SF-01-02-02) （2018ZDCXL-SF-01-02-02）
陕西高校青年创新团队(2019年) （2019年）
陕西省教育厅重点科学研究计划项目(20JY011、20JY012) （20JY011、20JY012）
