HPLC同时测定舒筋通络黑膏药9个成分含量OA北大核心CSTPCD
Simultaneous Determination of 9 Components in Shujin Tongluo Black Plaster by HPLC
目的 建立HPLC同时测定舒筋通络黑膏药中α-玉柏碱、阿魏酸、羟基红花黄色素A、苯甲酰新乌头原碱、苯甲酰乌头原碱、杠柳毒苷、4-甲氧基水杨醛、山柰酚、齐墩果酸含量的方法.方法 采用HPLC同时测定舒筋通络黑膏药70%甲醇提取液中9个成分,使用Waters SunFire C18色谱柱(4.6 mm×250 mm,5 μm),流动相为乙腈-0.2%磷酸水溶液,梯度洗脱,流速1.0 mL/min,柱温25℃,检测波长230 nm.结果 9个成分在各自范围内线性关系良好(r≥0.999 7),平均加样回收率为98.06%～100.56%,RSD为0.48%～2.56%.结论 本研究建立的方法重复性好,简便快速,可用于舒筋通络黑膏药的质量控制.
Objective To establish an HPLC-based method for the determination of α-obscurine,ferulic acid,hydroxysafflower yellow A,benzoylneaconitine,benzoylaconitine,periplosin,4-methoxysalicylsalicylate,kaempferol and oleanolic acid simultaneously in Shujin Tongluo Black Plaster.Methods HPLC method was used to determine the 9 components in Shujin Tongluo Black Plaster simultaneously.The 70%methanol extracts were analyzed using Waters SunFire C18 chromatography column(4.6 mm×250 mm,5 μm),in mobile phase containing acetonitrile-0.2%phosphoric acid solution for gradient elution with volume flow rate of 1.0 mL/min;column temperature was set at 25℃;detection wavelength was set at 230 nm.Results The 9 components had good linear relationship in their respective ranges(r≥0.999 7).The average recoveries were between 98.06%-100.56%and RSD was between 0.48%-2.56%,respectively.Conclusion The method has the advantage of repeatability,simple and fast,and can be used as the quality control of Shujin Tongluo Black Plaster.
王建良;李喜香;周亚丽;李小凤;苏秦;殷润良
甘肃中医药大学药学院,甘肃 兰州 730000甘肃省中医院药学部,甘肃 兰州 730050
中医学
舒筋通络黑膏药化学成分高效液相色谱法α-玉柏碱同时测定
Shujin Tongluo Black Plasterchemical compositionHPLCα-obscurinesimultaneous determination
《中国中医药信息杂志》 2024 (003)
138-143 / 6
甘肃省级重点人才项目-中药特色技术传承保护中心与人才培养基地(2021年)
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