中国中医药信息杂志2024，Vol.31Issue(3)：138-143,6.DOI:10.19879/j.cnki.1005-5304.202309561
HPLC同时测定舒筋通络黑膏药9个成分含量
Simultaneous Determination of 9 Components in Shujin Tongluo Black Plaster by HPLC
摘要
Abstract
Objective To establish an HPLC-based method for the determination of α-obscurine,ferulic acid,hydroxysafflower yellow A,benzoylneaconitine,benzoylaconitine,periplosin,4-methoxysalicylsalicylate,kaempferol and oleanolic acid simultaneously in Shujin Tongluo Black Plaster.Methods HPLC method was used to determine the 9 components in Shujin Tongluo Black Plaster simultaneously.The 70%methanol extracts were analyzed using Waters SunFire C18 chromatography column(4.6 mm×250 mm,5 μm),in mobile phase containing acetonitrile-0.2%phosphoric acid solution for gradient elution with volume flow rate of 1.0 mL/min;column temperature was set at 25℃;detection wavelength was set at 230 nm.Results The 9 components had good linear relationship in their respective ranges(r≥0.999 7).The average recoveries were between 98.06%-100.56%and RSD was between 0.48%-2.56%,respectively.Conclusion The method has the advantage of repeatability,simple and fast,and can be used as the quality control of Shujin Tongluo Black Plaster.关键词
舒筋通络黑膏药/化学成分/高效液相色谱法/α-玉柏碱/同时测定Key words
Shujin Tongluo Black Plaster/chemical composition/HPLC/α-obscurine/simultaneous determination分类
医药卫生引用本文复制引用
王建良,李喜香,周亚丽,李小凤,苏秦,殷润良..HPLC同时测定舒筋通络黑膏药9个成分含量[J].中国中医药信息杂志,2024,31(3):138-143,6.基金项目
甘肃省级重点人才项目-中药特色技术传承保护中心与人才培养基地(2021年) （2021年）
