中国真菌学杂志2023，Vol.18Issue(6)：493-500,8.
新疆某医院侵袭性念珠菌感染的菌种分布、药物敏感性及耐药基因初步分析
Preliminary analysis of strains distribution,drug susceptibility characteristics and resistance gene of invasive Candida infections in a hospital in Xinjiang
摘要
Abstract
Objective To investigate the distribution of strains,drug susceptibility characteristics,and azole resistance mechanisms of invasive Candida infections in a hospital in Xinjiang.Methods 125 strains isolated from clinically diagnosed invasive fungal infections patients were identified by fungal morphology and molecμlar biology to analyse the distribution of Candida isolates,the susceptibility characteristics of 9 antifungal drugs,and sequencing of ERG11 and PDR1 gene in azole-resistant/non-wide-type(NWT)Candida isolates.Results Of the 111 yeast strains successfully identified,the top three were Candida albicans(50.45%),Candida glabrata(18.02%)and Candida tropicalis(15.32%).Most fungal isolates were susceptible to amphotericin B,5-fluorocytosine and echinocandins,and only two strains of C.glabrata were resistant to echinocandins.Azoles had good in vitro susceptibility to C.albicans,while the antifungal activities of posaconazole and voriconazole against C.glabrata and C.tropicalis were low(＜60%).Missense mutations of ERG11 gene was found in 9 of 33 strains of azole-resistant Candida strains(6 C.albicans strains,14 C.glabra strains,11 C.tropicalis strains,1 C.krusei strain and 1 C.parapsilosis strain),and missense mutations of PDR1 gene was found in all azole-resistant C.gla-bra.strains.Conclusion C.albicans is the predominant causative agent of invasive Candida infections.Echinocandins,amphotericin B,and 5-FC show good in vitro antifungal activity against Candida isolates,and azoles are less effective a-gainst C.glabrata and C.tropicalis;azole resistance may be associated with the mutations of ERG11 and PDR1 gene.关键词
念珠菌/菌种分布/药敏特征/ERG11基因/PDR1基因/突变Key words
Candida/strains distribution/drug susceptibility characteristics/ERG11 gene/PDR1 gene/mutation分类
医药卫生引用本文复制引用
张松迪,王晓东,艾柯代·玉苏甫,哈地利亚·哈斯木,帕丽达·阿布利孜..新疆某医院侵袭性念珠菌感染的菌种分布、药物敏感性及耐药基因初步分析[J].中国真菌学杂志,2023,18(6):493-500,8.基金项目
国家自然科学基金(81960366) （81960366）
新疆维吾尔自治区自然科学基金(2021D01E30) （2021D01E30）
自治区重点研发计划项目(2021B03001-3) （2021B03001-3）
