中国真菌学杂志2023，Vol.18Issue(6)：511-517,7.
唑类耐药烟曲霉在江苏不同农田流行特点研究
Study on prevalence of azole resistance Aspergillus fumigatus isolated from different agriculture fields in Jiangsu province
摘要
Abstract
Objective We conducted a cross-sectional study to investigate hotspots environment and prevalence of azole-re-sistance Aspergillus fumigatus(RAF)isolates from agriculture fields in Jiangsu province.Methods Sixty-eight soil samples were taked in north and south area in Jiangsu province,Azole-resistant A.fumigatus were screened by selective medium and antifungal susceptibility test,cyp51A gene were amplified for detection of resistant mutation.Results Of 106 A.fu-migatus isolates recovered from 68 soil samples,34(32%)were azole resistant A.fumigatus.Eight of 34 had mutations in cyp51A gene(7 TR46/Y121 F/T289 A,1 TR34/L98 H/S297T/F495 I),the eight isolates were multi-resistant to itracon-azole,voriconazole and posaconazole,and have MIC＞16 mg/L to voriconazole.However,rest of 26 RAF isolates were not detected any mutation in cyp51A gene.Strawberry fields were detected the most frequently RAF isolates(16 in 34 isolates),followed by rice fields(8 in 34 isolates).RAF isolates in north Jiangsu were detected more frequently than those in south Jiangsu.RAF isolates from deeper soil were slightly more than those from superficial soil.Conclusion Our study results suggests that detection rate of RAF isolates in soil samples in Jiangsu agricultural fields was up to 32%,azole-resistance mechanism of A.fumigatus were mainly non-cyp51A gene related mechanism,Strawberry fields might be a potential hotspots for RAF.Managing the fungicides used in agriculture will help to control the emergence of antifungal drug resist-ance in clinics.关键词
江苏农田/唑类耐药/烟曲霉/流行特点Key words
agricultural fields in Jiangsu province/azole-resistance/Aspergillus fumigatus/prevalence分类
医药卫生引用本文复制引用
刘晨,黄瑞,胡丹阳,周益花,陆敏,邓淑文,史冬梅..唑类耐药烟曲霉在江苏不同农田流行特点研究[J].中国真菌学杂志,2023,18(6):511-517,7.基金项目
国家自然科学基金面上项目(NM82272358) （NM82272358）
苏州市科技计划项目(SKY2022037) （SKY2022037）
苏州高新区人民医院院管课题(SGY2019D02,SGY2021H01,SGY2021C01) （SGY2019D02,SGY2021H01,SGY2021C01）
