中国中医药信息杂志2024，Vol.31Issue(3)：26-33,8.DOI:10.19879/j.cnki.1005-5304.202307304
基于UPLC-Q-TOF-MS结合网络药理学与实验验证探讨健脾消癌方治疗结直肠癌作用机制
Discussion on the Mechanism of Jianpi Xiaoai Prescription in the Treatment of Colorectal Cancer Based on UPLC-Q-TOF-MS Combined with Network Pharmacology and Experimental Verification
摘要
Abstract
Objective To explore the active components of Jianpi Xiaoai Prescription and its mechanism in the treatment of colorectal cancer(CRC).Methods UPLC-Q-TOF-MS technology was used to identify the chemical components of Jianpi Xiaoai Prescription;Active components and target proteins were screened through TCMSP and SwissTargetPrediction databases;STRING database was used for protein interaction analysis;The GeneCards database was used to screen CRC-related targets;DAVID database was used for GO and KEGG pathway enrichment analysis;Molecular docking verification of main active components and key targets was conducted.A rat model of CRC was constructed,and the effect of Jianpi Xiaoai Prescription on key targets of CRC-related pathways in rats was verified by Western blot.Results Totally 35 active components of Jianpi Xiaoai Prescription,617 potential targets,and 325 intersected with CRC-related targets were obtained.The enrichment analysis of active component targets showed that the main enrichments were PI3K-Akt signaling pathway,EGFR tyrosine kinase inhibitors signaling pathway,T cell receptor signaling pathway,VEGF signaling pathway and other related pathways.13 key active components and 6 key proteins of EGFR,SRC,CA2,CA12,CYP19A1 and MAPK3 were obtained in Jianpi Xiaoai Prescription.The results of Western blot experiments showed that Jianpi Xiaoai Prescription could significantly reduce the protein expression levels of SRC and EGFR in rat CRC samples.Conclusion The active component in Jianpi Xiaoai Prescription may exert anti CRC cell proliferation,invasion,and metastasis effects by regulating EGFR,SRC with multiple targets and pathways.关键词
健脾消癌方/网络药理学/结直肠癌/超高效液相色谱-四极杆飞行时间串联质谱Key words
Jianpi Xiaoai Prescription/network pharmacology/colorectal cancer/UPLC-Q-TOF-MS分类
医药卫生引用本文复制引用
王莉,宋程,唐蔚..基于UPLC-Q-TOF-MS结合网络药理学与实验验证探讨健脾消癌方治疗结直肠癌作用机制[J].中国中医药信息杂志,2024,31(3):26-33,8.基金项目
湖南省自然科学基金(2022JJ30362、2021JJ40315) （2022JJ30362、2021JJ40315）
