中国中医药信息杂志2024，Vol.31Issue(3)：92-97,6.DOI:10.19879/j.cnki.1005-5304.202306008
电针对急性心肌缺血大鼠海马谷氨酸系统的影响
Effects of Electroacupuncture on the Hippocampus Glutamate System of Acute Myocardial Ischemia Rats
摘要
Abstract
Objective To observe the effects of electroacupuncture(EA)on glutamate(Glu),metabotropic glutamate receptor 2/3(mGluR2/3)and apoptosis related proteins expression in hippocampus in rats with acute myocardial ischemia(AMI);To explore the mechanism of EA against AMI.Methods Totally 50 SD rats were randomly divided into sham-operation group,model group,EA group and inhibitor group,with 10 rats in each group.Except for the sham-operation group,the rats were treated with ligation at the left anterior descending coronary artery to establish AMI model.The rats in the EA group was treated with EA at"Shenmen"and"Tongli",30 minutes each time,once a day for 3 consecutive days.The rats in the inhibitor group were treated with injection of LY341459 via the lateral ventricle 30 min after modeling.HE staining was used to observe myocardial tissue morphology,and ELISA was used to detect Caspase-3 activity in myocardial tissue and Glu content in hippocampal tissue,immunofluorescence staining was used to detect mGluR2/3 expression in hippocampal tissue,TUNEL staining was used to detect apoptosis in hippocampal tissue cells,Western blot was used to detect the expressions of PI3K,Akt,and Caspase-3 protein in hippocampal tissue.Results Compared with the sham-operation group,the myocardial cells of the model group rats showed sparse and swelling with severe infiltration of inflammatory cells;the activity of Caspase-3 in myocardial tissue significantly increased,and the Glu content,positive expression of mGluR2/3,number of apoptotic cells in hippocampal tissue significantly increased(P<0.01),and the expressions of PI3K and Akt proteins in hippocampal tissue were significantly decreased,while the expression of Caspase-3 protein significantly increased(P<0.01).Compared with the model group,myocardial cell edema and inflammatory cell infiltration were reduced in the EA group and inhibitor group,the activity of Caspase-3 in myocardial tissue was significantly decreased,the Glu content,positive expression of mGluR2/3,and number of apoptotic cells in hippocampal tissue were significantly reduced(P<0.01),the expressions of PI3K and Akt proteins in hippocampal tissue significantly increased,while the expression of Caspase-3 protein significantly decreased(P<0.01).Conclusion EA can improve myocardial injury in AMI rats,and its mechanism may be related to activation of PI3K/Akt signaling pathway,inhibition of hippocampal mGluR2/3 overexpression,reduction of Glu accumulation,inhibition of apoptosis of hippocampal neurons and reduction of neurotoxicity.关键词
急性心肌缺血/电针/海马/谷氨酸/代谢型谷氨酸受体2/3/大鼠Key words
acute myocardial ischemia/electroacupuncture/hippocampus/Glu/metabotropic glutamate receptors 2/3/rats分类
医药卫生引用本文复制引用
童思佳,王堃,吴生兵,周美启..电针对急性心肌缺血大鼠海马谷氨酸系统的影响[J].中国中医药信息杂志,2024,31(3):92-97,6.基金项目
国家自然科学基金(82004462) （82004462）
安徽省中医药领军人才建设项目(2019年) （2019年）
浙江中医药大学科研开放基金(ZYXYB2019002) （ZYXYB2019002）
