中国农业科技导报2024，Vol.26Issue(3)：117-123,7.DOI:10.13304/j.nykjdb.2023.0124
海南省东方市甜瓜细菌性果斑病病原鉴定
Identification of Pathogen Causing Bacterial Fruit Blotch of Melon in Hainan
摘要
Abstract
To identify the pathogen which seriously caused fruit blotch disease in melo,the melon fruit samples suspected bacterial fruit blotch disease were collected from Dongfang city,Hainan province.Pathogen was isolated and purified from samples,and the pathogenicity of bacterium strains was confirmed according to Koch's postulate.The identification of the pathogenic strains was carried out according to the colony morphology,physiological and biochemical analysis and 16S rDNA sequences analysis.The results showed that the isolated bacterium with similar cultural characteristics was obtained from all diseased samples,which the separation rate of bacteria was 100％.All of tested strains showed pathogenic to melon fruits.Strains were identified as Gram-negative bacterium,which colony morphology,physiology and biochemistry shared similarly with that of Acidovorax citrull.BLAST analysis showed that the 16S rDNA sequences had 100％homology with that of A.citrulli,and the phylogenetic analysis showed the pathogen was A.citrulli.Above results determined the pathogen of melon fruit blotch,which provided a basis for effective prevention and control of this disease.关键词
甜瓜/细菌性果斑病/病原菌鉴定/西瓜嗜酸菌/海南省东方市Key words
melon/bacterial fruit blotch/pathogen identification/Acidovorax citrulli/Dongfang city in Hainan province分类
农业科技引用本文复制引用
李鹏声,黄清泰,范咏梅,王萌,杨叶..海南省东方市甜瓜细菌性果斑病病原鉴定[J].中国农业科技导报,2024,26(3):117-123,7.基金项目
海南省自然科学基金项目(321RC457) （321RC457）
海南省东方市农业绿色发展先行先试支撑体系建设项目(20210714). （20210714）
