中国中医药信息杂志2024，Vol.31Issue(3)：98-104,7.DOI:10.19879/j.cnki.1005-5304.202303457
百事乐胶囊调控SHH/Gli1信号通路对抑郁模型大鼠海马神经再生的影响
Effects of Regulation of SHH/Gli1 Signaling Pathway by Baishile Capsules on Hippocampal Neurogenesis Depression Model Rats
摘要
Abstract
Objective To explore the effects and mechanism of Baishile Capsules regulating SHH/Gli1 signaling pathway on hippocampal neurogenesis of depression model rats.Methods Totally 32 SD rats were randomly divided into control group,model group,fluoxetine(5.4 mg/kg)group and Baishile Capsules(2.88 g/kg)group,with 8 rats in each group.A depression rat model was established using chronic unpredictable mild stress and single cage feeding method.The model was established and administered simultaneously for 21 consecutive days.Depression-like behavior in rats were evaluated by sucrose preference experiment and open field experiment,ELISA was used to detect brain derived neurotrophic factor(BDNF)contents in rat serum and hippocampal tissue,the number of BrdU,BrdU/DCX,BrdU/NeuN positive cells in dentate gyrus of the hippocampus was observed by immunofluorescence,immunofluorescence and Western blot were used to detect the fluorescence intensity and protein expression of SHH,Gli1,Smo,Ptch in hippocampal tissue.Results Compared with the control group,the degree of sucrose preference significantly decreased in the model group(P<0.01),the number of horizontal and vertical movements significantly decreased(P<0.01),the contents of BDNF in serum and hippocampal tissue significantly decreased(P<0.05),the number of BrdU,BrdU/DCX,BrdU/NeuN positive cells in dentate gyrus of the hippocampus significantly decreased(P<0.01),and the fluorescence intensity and protein expression of SHH,Gli1,Smo,Ptch in hippocampal tissue significantly decreased(P<0.01,P<0.05).Compared with the model group,the degree of sucrose preference and the number of horizontal and vertical movements in fluoxetine group and Baishile Capsule group increased significantly(P<0.05,P<0.01),the contents of BDNF in serum and hippocampal tissue significantly increased(P<0.05,P<0.01),and the number of BrdU,BrdU/DCX,BrdU/NeuN positive cells in dentate gyrus of the hippocampus significantly increased(P<0.01,P<0.05),the fluorescence intensity and protein expressions of SHH,Gli1,Smo,Ptch in hippocampal tissue significantly increased(P<0.01,P<0.05).Conclusion Baishile Capsule can promote the hippocampus neurogenesis in depression model rats by regulating SHH/Gli1 signaling pathway,and play an antidepressant role.关键词
百事乐胶囊/抑郁症/海马/SHH/Gli1信号通路/脑源性神经营养因子/神经再生Key words
Baishile Capsules/depression/hippocampus/SHH/Gli1 signaling pathway/BDNF/neurogenesis分类
医药卫生引用本文复制引用
骆妍,蔡川,侯光菡,蔺晓源,杨蕙,吴梅,孟盼..百事乐胶囊调控SHH/Gli1信号通路对抑郁模型大鼠海马神经再生的影响[J].中国中医药信息杂志,2024,31(3):98-104,7.基金项目
湖南省自然科学基金优秀青年基金(2020JJ3027) （2020JJ3027）
湖南省科技创新人才计划优秀博士后创新人才项目(2020RC2060) （2020RC2060）
湖南省中医药科研课题(B2023021) （B2023021）
湖南省教育厅科学研究项目(18C0372) （18C0372）
长沙市杰出创新青年培养计划(kq2009018) （kq2009018）
长沙市自然科学基金(kq2208471) （kq2208471）
