中国中医药信息杂志2024，Vol.31Issue(3)：119-126,8.DOI:10.19879/j.cnki.1005-5304.202304482
冠心病心血瘀阻证大鼠心肌组织代谢组学研究
Study on Metabonomics of Myocardial Tissue of Rat Model with Coronary Heart Disease of Heart Blood Stasis Syndrome
摘要
Abstract
Objective To investigate the biological basis of disease and syndrome by studying the spectrum of myocardial tissue metabolites in the rat model of coronary heart disease with heart blood stasis syndrome.Methods SD rats were randomly divided into sham-operation group and model group.The left anterior descending coronary artery was ligated to prepare the rat model of coronary heart disease with heart blood stasis syndrome.The general condition was observed,and the tongue chromaticity,electrocardiogram,cardiac function were detected.HE staining and transmission electron microscopy were used to observe myocardial tissue morphology and ultrastructure.UPLC-MS technology was used to investigate the differential metabolites in rat myocardial tissue,and enrichment analysis was conducted on metabolic pathways.Results Compared with the sham-operation group,the tongue chromaticity R,G,B values of model group rats were significantly reduced(P<0.05),ECG heart rate and ST segment elevation amplitude significantly increased(P<0.05),LVEF and LVFS significantly decreased,and LVIDs and LVIDd significantly increased(P<0.05).Myocardial tissue pathology revealed that the structure was blurred,inflammatory cells infiltrated,mitochondria swelled,ruptured,and dissolved,and crista structure fracture decreased.A total of 29 potential biomarkers with significant differences between the sham-operation group and the model group were identified in metabolomics(7 upregulated and 22 downregulated),with the majority of 10 pathways enriched in thiamine metabolism,arginine biosynthesis,purine metabolism,aminoacyl-tRNA biosynthesis,alanine,aspartate and glutamate metabolism,pentose and glucuronate interconversions,glycolysis/gluconeogenesis,valine,leucine and isoleucine degradation,TCA cycle,pyruvate metabolism.Conclusion Ligation of the left anterior descending coronary artery can mimic the pathological process of coronary heart disease with blood stasis syndrome in a good way,and its pathological mechanism involves the disruption of multi-level metabolic networks such as glucose metabolism,mitochondrial energy metabolism,amino acid metabolism,protein biosynthesis,and purine metabolism.关键词
冠心病/心血瘀阻证/生物学基础/代谢组学/大鼠Key words
coronary heart disease/heart blood stasis syndrome/biological basis/metabonomics/rats分类
医药卫生引用本文复制引用
李静,郭志华,刘建和,钟森杰,匡慧芳,杨漾,刘祎,张秋雁..冠心病心血瘀阻证大鼠心肌组织代谢组学研究[J].中国中医药信息杂志,2024,31(3):119-126,8.基金项目
国家自然科学基金(81574039、82174343) （81574039、82174343）
湖南省教育厅重点项目(21A0253) （21A0253）
湖南中医药大学学科建设"揭榜挂帅"项目(22JBZ005) （22JBZ005）
中药粉体与创新药物省部共建国家重点实验室培育基地开放基金项目(21PTKF1012) （21PTKF1012）
湖南中医药大学研究生创新项目(2022CX04) （2022CX04）
