中国农业科技导报2024，Vol.26Issue(3)：214-222,9.DOI:10.13304/j.nykjdb.2022.0769
载银氧化壳聚糖的制备与抑菌性能研究
Research on Antibacterial Activity of Silver-Carried Oxidized Chitosan
摘要
Abstract
In order to prepare a highly effective antibacterial agent,chitosan(CS)was used as raw material to prepare 6-carboxyl chitosan(C-COS)by laccase/2,2,6,6-tetramethylpiperidine-1-oxyl radical(TEMPO)system,and then the silver-carried oxidized chitosan(C-COS-Ag)was prepared by reaction with silver nitrate.The antibacterial activity of C-COS-Ag was studied.The results showed that the antibacterial effect of C-COS-Ag was significantly improved compared with CS.Its inhibitory zone diameters against Escherichia coli and Staphylococcus aureus were(22.75±1.50)and(13.75±2.50)mm,respectively,with a minimum inhibitory concentration of 6.10 μg·mL-1.The bacterial growth curve test confirmed that C-COS-Ag could significantly reduce the growth rate of Escherichia coli and Staphylococcus aureus;the penetration test of cell contents showed that C-COS-Ag could destroy the cell membrane and cell wall morphology of bacteria,resulting in the exudation of nucleic acids,proteins and other macromolecules,the increase of alkaline phosphatase activity,the decrease of β-galactosidase activity,the obstruction of galactose synthesis pathway,and the normal metabolism of bacteria were affected.Above results provided a theoretical basis for the application of C-COS-Ag.关键词
壳聚糖/硝酸银/抑菌性/大肠杆菌/金黄色葡萄球菌Key words
chitosan/silver nitrate/antiacterstasis/Escherichia coil/Staphylococcus aureus分类
轻工纺织引用本文复制引用
纪蕾,刘天红,王颖,李晓,李红艳,孙元芹,姜晓东..载银氧化壳聚糖的制备与抑菌性能研究[J].中国农业科技导报,2024,26(3):214-222,9.基金项目
山东省现代农业虾蟹产业技术体系项目(SDAIT-13-07). （SDAIT-13-07）
