中国水产科学2023，Vol.30Issue(9)：1080-1092,13.DOI:10.12264/JFSC2023-0158
短期高脂应激对珍珠龙胆石斑鱼幼鱼生长性能、肝脏健康和脂代谢基因的影响
Effects of short-term high-fat stress on growth performance,liver health,and lipid metabolism genes in Epinephelus fuscoguttatus ♀ × E.lanceolatus ♂
摘要
Abstract
The aim of this experiment was to investigate the effects of high-fat diets on the short-term growth performance and antioxidant capacity of pearl grouper to help formulators and farmers anticipate the possible negative effects of high-fat diets on grouper growth and take effective measures in the formulation and feeding schedule of high-fat diets.Seven groups of isonitrogenous diets with fat levels of 8％(EE8),10％(EE10),12％(EE12),14％(EE14),16％(EE16),18％(EE18),and 20％(EE20)were fed to pearl grouper(initial mean weight:13.70±0.01g)for 4 weeks with a crude protein level of 48％.The experimental results showed no significant change in weight gain rate(WGR)and survival rate(SR)as the level of dietary ether extract increased(P＞0.05),while the feed intake was significantly lower in the EE14-EE20 group(P＜0.05);further,the viscerosomatic index(VSI)and protein efficiency ratio(PER)increased significantly(P＜0.05)after the dietary ether extract exceeded 16％.The EE14-EE20 group had a significantly higher abdominal muscle ether extract(AMEEC)(P＜0.05),and the EE16-EE20 group had a significantly greater hepatosomatic index(HSI)(P＜0.05).Compared to the EE12 group,total cholesterol(T-CHO)was significantly higher in the EE8,EE10,EE18,and EE20 groups(P＜0.05);glutathione peroxidase(GSH-PX)activity was significantly lower in the EE16-EE20 group(P＜0.05);malondialdehyde(MDA)content was significantly higher in the EE20 group(P＜0.05);and vacuolated area and hepatocytes in the EE8 and EE16-EE20 groups'lipid droplet amount were significantly increased(P＜0.05).The relative expression of the lipid synthesis genes g6pd,6gpd,and me was significantly increased in the EE20 group(P＜0.05),and the lipolytic genes cpt-1 and pparα were significantly increased in the EE16-EE20 group(P＜0.05).The results have shown that dietary ether extract above 16.77％causes fatty deposits in the liver of pearl amberjack,reducing the liver's antioxidant capacity and impairing liver health.关键词
珍珠龙胆石斑鱼/高脂饲料/生长性能/抗氧化能力/脂质代谢Key words
Epinephelus fuscoguttatus ♀ × E.lanceolatus ♂/high-fat diet/growth performance/antioxidant capacity/Lipid metabolism分类
农业科技引用本文复制引用
廖春燕,高文浩,李嘉辉,迟淑艳..短期高脂应激对珍珠龙胆石斑鱼幼鱼生长性能、肝脏健康和脂代谢基因的影响[J].中国水产科学,2023,30(9):1080-1092,13.基金项目
广东省基础与应用基础研究基金项目(2021A1515010634). （2021A1515010634）
